CLDN 3通过参与氧化磷酸化途径促进子宫内膜癌细胞增殖  

CLDN 3 promotes the proliferation of endometrial cancer cells by participating in oxidative phosphorylation pathway

作　　者：张林静 李常虹 胡月新 黄芩[3] 韦焘[3] ZHANG Linjing;LI Changhong;HU Yuexin;HUANG Qin;WEI Tao(Department of Obstetrics and Gynecology,Hainan Women And Children's Medical Center,Haikou Hainan 570206;Research and Experimental Center,Kunming Medical University;Kunming Medical University,Kunming Yunan 650500,P.R.China)

机构地区：[1]海南省妇女儿童医学中心妇产科,海南海口570206 [2]昆明医科大学科研实验中心,云南昆明650500 [3]昆明医科大学,云南昆明650500

出　　处：《中国计划生育和妇产科》2020年第9期84-90,共7页Chinese Journal of Family Planning & Gynecotokology

摘　　要：目的研究CLDN 3基因在子宫内膜癌发生发展中的生物学功能和潜在分子机制。方法通过临床数据库GEPIA查找并确定在子宫内膜癌组织中具有显著差异表达及其临床预后目的的基因;通过siRNA介导敲低目的基因的表达,利用细胞增殖实验和克隆形成实验验证目的基因在子宫内膜癌发生发展中的作用;探索目的基因在子宫内膜癌中发挥功能的潜在分子机制,通过GEPIA数据库查看关键基因在子宫内膜癌中的表达特征;T-qPCR检测目的基因对信号通路关键基因的调控,细胞增殖实验验证关键基因在子宫内膜癌发生发展中的作用。结果GEPIA数据库显示CLDN 3在子宫内膜癌中显著高表达(P<0.05)且高表达预后差(P<0.05);细胞增殖实验发现,在敲低CLCN 3表达的子宫内膜癌细胞中,与对照组siCTL相比,细胞增殖速率显著减慢(P<0.05);克隆形成实验同样发现,在敲低CLDN 3表达的子宫内膜癌细胞中,与对照组siCTL相比,克隆形成速率显著减慢(P<0.05);GEPIA数据库显示氧化磷酸化关键基因NDUFA 2,NDUFB 9,COX 7 B,COX 6 B 1,NDUFA 13,COX 5 B,ATP 6 V 0 B均在子宫内膜癌中显著高表达(P<0.05);RT-qPCR实验显示在子宫内膜癌细胞中敲低CLDN 3的表达,氧化磷酸化关键基因的表达被降低(P<0.05);细胞增殖实验发现,在敲低氧化磷酸化关键基因表达的子宫内膜癌细胞中,与对照组siCTL相比,细胞增殖速率显著减慢(P<0.05)。KEGG信号通路预测CLDN 3可能参与氧化磷酸化途径。结论CLDN 3在子宫内膜癌中高表达,其可能通过正调控氧化磷酸化关键基因的高表达,从而促进子宫内膜癌的生长。Objective To study the biological function and potential molecular mechanism of CLDN 3 gene in the development of endometrial carcinoma.Methods The target genes with significant differential expression in endometrial carcinoma and their clinical prognosis were identified by using the clinical database GEPIA.The role of target genes in the development of endometrial carcinoma was verified by siRNA mediated knockdown of target genes through cell proliferation assay and cloning formation assay.To explore the potential molecular mechanism by which the target gene plays a role in endometrial cancer,and to investigate the expression characteristics of key genes in endometrial cancer through GEPIA database.RT-qPCR was used to detect the regulation of target genes on key genes in the signaling pathway,and cell proliferation assay verified the role of key genes in the development of endometrial cancer.Results The GEPIA database showed that CLDN 3 was significantly overexpressed in endometrial cancer and the overexpressed CLDN 3 had poor prognosis(P<0.05).The cell proliferation assay found that in the endometrial cancer cells with low expression of CLCN 3,the cell proliferation rate was significantly slower than in the control group siCTL(P<0.05).The cloning formation experiment also found that in the endometrial cancer cells with low CLDN 3 expression,the cloning formation rate slowed down significantly compared with the control group siCTL(P<0.05).The GEPIA database showed that the key oxidative phosphorylation genes NDUFA 2,NDUFB 9,COX 7 B,COX 6 B 1,NDUFA 13,COX 5 B and ATP 6 V 0 B were all highly expressed in endometrial carcinoma(P<0.05).RT-qPCR showed that the expression of CLDN 3 was knocked down in endometrial cancer cells,and the expression of key oxidative phosphorylation genes was reduced(P<0.05).The cell proliferation assay found that the proliferation rate of endometrial cancer cells was significantly slower than that of the control group siCTL(P<0.05).The KEGG signaling pathway predicted that CLDN 3 may parti

关 键 词：子宫内膜癌 CLDN 3 KEGG信号通路 

分 类 号：R737.33[医药卫生—肿瘤]