野外条件下球虫感染对根田鼠免疫功能的影响  

The effect of coccidian infection on immune function of root voles (Microtus oeconomus) :a field experiment

作　　者：尚国珍 吴学琴 朱亚辉 曹伊凡[1,4] 吴雁 边疆晖[1,4] SHANG Guozhen;WU Xueqin;ZHU Yahui;CAO Yifan;WU Yan;BIAN Jianghui(Key Laboratory of Adaptation and Evolution of Plateau,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001,China;University of Chinese Academy of Sciences,Beijing 100049,China;School of Life and Environment Sciences,Hangzhou Normal University,Hangzhou 310012,China;Qinghai Key Laboratory of Animal Ecological Genomics,Xining 810001,China)

机构地区：[1]中国科学院西北高原生物研究所,高原生物适应与进化重点实验室,西宁810001 [2]中国科学院大学,北京100049 [3]杭州师范大学生命与环境科学学院,杭州310012 [4]青海省动物生态基因组学重点实验室,西宁810001

出　　处：《兽类学报》2020年第5期413-423,共11页Acta Theriologica Sinica

基　　金：国家科技重点研发计划(2016YFC0501901);国家自然科学基金项目(31570421);中国科学院A类先导专项(XDA2005010406)。

摘　　要：动物的免疫功能是影响动物适合度的重要因素之一。本文以根田鼠(Microtus oeconomus)为研究对象,在野外围栏条件下,通过球虫感染和混合驱虫药处理,测定根田鼠的植物血凝素反应(Phytohemagglutinin,PHA)、血清抗匙孔血蓝蛋白(Keyhole limpet haemoeyanin,KLH)的IgG水平和血象参数,探讨寄生物感染对免疫功能的影响。结果表明,根田鼠随球虫感染率和感染强度的增加,对PHA的反应峰值降低、血清抗KLH的IgG水平、白细胞总数、淋巴细胞的百分比及血浆蛋白含量明显下降、嗜碱性粒细胞的百分比增加,嗜酸性粒细胞、嗜中性粒细胞和单核细胞的百分比未检出显著变化。由此说明,球虫感染降低了根田鼠的细胞免疫和体液免疫功能。The immune function of animals is an important factor to affect the fitness of animal.In this study,we measured phytohemagglutinin(PHA)response,anti-keyhole limpet hemocyanin immunoglobulin G(anti-KLH IgG)level and hematological parameters of root voles(Microtus oeconomus)by controlling infection of coccidioides in the enclosure to study the effect of coccidian infection on immune function.Our results show that coccidian infection treatment significantly increased the prevalence and intensity of coccidia during the experiment,and significantly reduced the PHA response,serum anti-KLH IgG levels,white leukocyte counts,the percentage of lymphocytes,and plasma protein concentration in root vole.And infection treatment significantly increased the percentage of basophils,the significant difference between both treatments in percentage of eosinophils,neutrophils and monocytes were not found.These results indicate that coccidian infection has negative effects on the cell mediated immune response and humoral immune response of root voles.

关 键 词：寄生物 免疫功能 球虫 种群调节 根田鼠 

分 类 号：Q494[生物学—生理学]