腺嘌呤核苷三磷酸生物发光法快速检测短乳杆菌  

Rapid detection of Lactobacillus brevis in beer with ATP bioluminescence

作　　者：郭立芸 向杰 谢鑫 侍亚敏 GUO Liyun;XIANG Jie;XIE Xin;SHI Yamin(Technology Center,Beijing Yanjing Beer Co.,Ltd.,Beijing 101300,China;Key Laboratory of Beer Brewing Technology in Beijing,Beijing 101300,China)

机构地区：[1]北京燕京啤酒股份有限公司技术中心,北京101300 [2]啤酒酿造技术北京市重点实验室,北京101300

出　　处：《食品与发酵工业》2020年第18期232-235,共4页Food and Fermentation Industries

摘　　要：啤酒酿造过程中的有害微生物及有机质残留检测与控制是防止微生物污染、保障啤酒质量的主要控制因素。该文以腺嘌呤核苷三磷酸(adenosine triphosphate,ATP)生物发光法为基础,建立啤酒酿造过程中微生物污染风险的快速评估方法。采用便携式ATP荧光检测仪对表面人工污染的短乳杆菌、梯度稀释的短乳杆菌菌悬液和啤酒有机质残留进行检测,分别对相对荧光单位(relative luciferase unit,RLU)和表面细菌总数、细菌浓度和啤酒有机质残留进行相关性分析,并建立预测模型。结果显示,表面细菌总数、水质细菌浓度和啤酒有机质残留预测模型的相关系数均>0.9(P<0.05),其中啤酒有机质残留预测模型的相关系数最高(R 2=0.994)。基于便携式ATP快速荧光检测系统建立的表面短乳杆菌总数、水质短乳杆菌浓度和啤酒有机质残留预测模型,可对啤酒酿造过程中卫生学检验提供有效的实时监控,也为ATP生物发光法在食品质量安全的应用提供了理论支撑。The detection and control of beer spoilage and organic matter residues in beer brewing process is one of the main effective methods to prevent microbial contamination and ensure beer quality.This study aimed to establish a rapid assessment method of microbial contamination risk during beer brewing based on the adenosine triphosphate(ATP)bioluminescence method.A portable ATP fluorescence detector was used to detect the contamination of Lactobacillus brevis on the surface,in the gradient diluted L.brevis suspension and the residual organic matter in beer.The correlation analysis of the relative fluorescence unit(RLU)and the total number of L.brevis on the surface,bacterial concentration in water and beer organic matter residues was conducted,respectively.Three kinds of different prediction models were established.The correlation coefficients of these“matched”combinations including total surface bacteria,bacterial concentration in water and beer organic matter residual prediction model,were all greater than 0.9(P<0.05).Among them,beer organic matter residual prediction model was highly relevant to RLU(R 2=0.994).The prediction model of total L.brevis on the surface,concentration of L.brevis in water and residual organic matter in beer was established based on the portable ATP fast fluorescence detection system,which can provide effective real-time monitoring for the hygiene inspection in the beer brewing process,and also provides theoretical support for the application of luminescence method in food quality and safety.

关 键 词：ATP生物发光法 表面细菌总数 水质细菌浓度 预测模型 

分 类 号：TS262.5[轻工技术与工程—发酵工程]