己糖激酶2促进人宫颈癌细胞系的迁移与增殖  

HK2 promotes migration and proliferation of human cervical cancer cell lines

作　　者：雷丹 张燕茹[1] 陈茜[1] 李露 崔南 LEI Dan;ZHANG Yan-ru;CHEN Qian;LI Lu;CUI Nan(Department of Reproductive Medicine,the First Affiliated Hospital of Xi an Jiaotong University,Medical College of Xi an Jiaotong University,Xi an 710061;Department of Nephrology,the First Affiliated Hospital of Xi an Medical University,Xi an 710021,China)

机构地区：[1]西安交通大学第一附属医院生殖医学科,西安交通大学医学部,陕西西安710061 [2]西安医学院第一附属医院肾病科,陕西西安710021

出　　处：《基础医学与临床》2020年第10期1320-1327,共8页Basic and Clinical Medicine

基　　金：国家自然科学基金(81903042)。

摘　　要：目的研究己糖激酶2(HK2)通过p-ERK1/2促进人子宫颈癌细胞HeLa增殖和迁移的分子机制。方法用Western blot和细胞免疫化学检测HK2在宫颈癌细胞系中的表达;用G418压力筛选获取HK2稳定表达的HeLa-HK2及对照细胞系;用细胞划痕和Transwell小室法检测HeLa-HK2及对照细胞的迁移和侵袭;用细胞计数、MTT法、流式细胞仪检测HeLa-HK2及对照细胞的增殖、细胞活力及细胞周期的分布;用Western blot和细胞免疫化学检测ERK1/2、p-ERK1/2、cyclin A和Slug蛋白的表达;用ERK抑制剂(FR180204)抑制ERK1/2和p-ERK1/2表达后观察HeLa-HK2细胞增殖、迁移和侵袭能力的变化。结果成功构建HK2稳定表达的HeLa-HK2细胞系;HeLa-HK2细胞迁移、侵袭及增殖能力显著强于对照细胞,HeLa-HK2细胞中p-ERK1/2、cyclin A和Slug蛋白表达高于对照细胞;使用FR180204在HeLa-HK2细胞中抑制ERK1/2和p-ERK1/2表达后,可以抑制HK2对细胞增殖、迁移和侵袭的增强作用。结论HK2通过p-ERK1/2上调cyclin A和Slug蛋白表达促进细胞的增殖、迁移和侵袭。Objective To investigate the molecular mechanism of HK2 on promoting cell proliferation and migration through p-ERK1/2 in cervical cancer cell line HeLa.Methods Expression of HK2 in cervical cancer cell lines was detected by Western blot and immunocychemistry.Over-expressed HK2 cell line(HeLa-HK2)was established by using G418.Wound-healing and Transwell cells assays were used to detect the migratory and invasive potential in HeLa-HK2 and in control cell lines.Cell growth curves,MTT assay and flow cytometry were used to detect cell proliferation,cell viability and cell cycle.The expression of ERK1/2,p-ERK1/2,cyclin A and Slug was detected by using Western blot and immunocytochemistry.The ERK inhibitor(FR180204)was used to inhibit expression of ERK1/2 and p-ERK1/2,and the potential of cell proliferation,migration and invasion was observed in these cells.Results HeLa-HK2 cells that stably expressing HK2 was successfully constructed.HK2 enhanced cell migration,invasion and growth potential in HeLa cells.The expression of ERK1/2,p-ERK1/2,cyclin A and Slug was higher in HeLa-HK2 cells than those in control cells.The protein level of ERK1/2,p-ERK1/2,cyclin A and Slug was decreased in FR180204 treated HeLa-HK2 cells,and the potential of growth,migratory and invasive were also attenuated by FR180204 in HeLa-HK2 cells.Conclusions HK2 promotes cell growth,migration and invasion in HeLa cells by up-regulating expression of cyclin A and Slug through stimulating p-ERK1/2.

关 键 词：己糖激酶2 P-ERK1/2 SLUG cyclin A 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]