不同产地乌饭树叶中黄酮成分含量差异研究  

Analysis of flavonoids in the leaves of Vaccinium bracteatum Thunb.from different regions

作　　者：黄婧[1] 赵青[2] 周鹏[1] 张敏[1] Huang Jing;Zhao Qing;Zhou Peng;Zhang Min(Jiangsu Academy of Forestry,Nanjing 211153,China;Nanjing Forestry University,Nanjing 210037,China)

机构地区：[1]江苏省林业科学研究院,江苏南京211153 [2]南京林业大学,江苏南京210037

出　　处：《江苏林业科技》2020年第4期8-12,21,共6页Journal of Jiangsu Forestry Science & Technology

基　　金：江苏省林业科学研究院青年基金项目“基于SNP标记技术的乌饭树群体遗传多样性研究”(2016-JFA-007);中央财政林业科技推广示范资金项目“乌饭树良种繁育及栽培技术推广”(苏[2019]TG02)。

摘　　要：对湖南白云山、安徽黄山、江西上犹、江苏溧阳、安徽宣城5个种源乌饭树叶的主要生物活性成分黄酮进行提取与分析,评价不同产地乌饭树叶综合品质,为乌饭树资源开发利用提供理论依据。采用三氯化铝显色法和高效液相色谱法测定其叶中的总黄酮含量及槲皮素、木犀草素、山柰酚和芹菜素含量。5个产区总黄酮含量为5.994—17.942 mg/g,槲皮素含量为0.776—6.490 mg/g,木犀草素为0.012—0.036 mg/g,山柰酚含量为0.006—1.350 mg/g,芹菜素含量为0.014—0.191 mg/g,不同产地叶片的各成分含量之间存在显著差异(P<0.05);利用模糊数学中隶属函数分析法对各产地的乌饭树叶综合品质进行了评价,认为综合品质最好的为源自江苏溧阳的乌饭树叶,较好的为江西上犹和安徽宣城生长的乌饭树叶,湖南白云山和安徽黄山的乌饭树叶综合品质较差。In order to lay a theoretical foundation for selection and development of genetic resources in Vaccinium bracteatum Thunb.,the flavonoids content was determined by the aluminium trichloride chromogenic method,and the contents of quercetin,luteolin,kaempferol and apigenin were detected by HPLC method.The results indicated that total amount of flavonoids was 5.994—17.942 mg/g,content of quercetin was 0.776—6.490 mg/g,luteolin content was 0.012—0.036 mg/g,kaempferol was 0.006—1.350 mg/g,apigenin was 0.014—0.191 mg/g,with significant differences between the leaves from different provonances(P<0.05).The evaluation of comprehensive quality of V.Bracteatum from different provonances showed that the quality of the leaves from Liyang,Jiangsu was the best,those from Shangyou,Jiangxi and Xuancheng,Anhui were better,and those from Mount Baiyun in Hunan and Mount Huang in Anhui’were poor.

关 键 词：乌饭树 类黄酮 槲皮素 综合评价 产地 

分 类 号：Q946.833[生物学—植物学] Q949.9