人肝癌HepG2细胞miR-26a对其活性和Wnt信号通路的作用机制  

Mechanism of Human liver cancer HepG2 cells miR-26a on its activity and Wnt signaling pathway

作　　者：李杰[1] 马莉[1] 贾丽君[1] 梁亮[1] 昝瑛[1] LI Jie;MA Li;JIA Lijun;LIANG Liang;ZAN Ying(Department of Oncology,The Second Affiliated Hospital of Xi’an Jiaotong University,Xi'an 710004,China)

机构地区：[1]西安交通大学第二附属医院肿瘤科,陕西西安710004

出　　处：《西部医学》2020年第9期1254-1258,共5页Medical Journal of West China

基　　金：陕西省重点研发计划项目(2019SF-042)。

摘　　要：目的探讨人肝癌HepG2细胞miR-26a对其活性和Wnt信号通路的作用机制。方法将人肝癌细胞株HepG2细胞分为肝癌组(无干预的HepG2细胞)、miR-26a组(HepG2细胞转染miR-26aminmics)(模拟物)、miR-26a-NC组(HepG2细胞转染miR-26a-NC)(空转),多种方法检测HepG2生物活性,免疫印迹和PCR分别检测β-catenin、Wnt3蛋白水平和mRNA表达。结果miR-26a-NC组与肝癌组HepG2细胞侵袭数量、HepG2细胞迁移率、HepG2细胞凋亡率,两组比较差异无统计学意义(P>0.05),miR-26a组HepG2细胞侵袭数量、HepG2细胞迁移率、HepG2细胞凋亡率,与miR-26a-NC组相比差异有统计学意义(P<0.05);miR-26a-NC组与肝癌组HepG2细胞中β-catenin、Wnt3蛋白水平比较差异无统计学意义(P>0.05),miR-26a组HepG2细胞β-catenin、Wnt3蛋白水平与miR-26a-NC组比较差异有统计学意义(P<0.05);miR-26a-NC组与肝癌组HepG2细胞中β-catenin、Wnt3mRNA水平比较差异无统计学意义(P>0.05),miR-26a组HepG2细胞β-catenin、Wnt3mRNA水平与miR-26a-NC组比较差异有统计学意义(P<0.05)。结论miR-26a抑制HepG2细胞活性,促使其凋亡,其作用机制可能与抑制β-catenin、Wnt3表达相关。Objective To investigate the mechanism of miR 26a on its activity and Wnt signaling pathway in human hepatoma HepG2 cells.Methods The human liver cancer cell line HepG2 cells were divided into liver cancer group(HepG2 cells without intervention),miR-26a group(intervention of HepG2 cells with miR-26a mimics)and miR-26a-NC group(using miR-26a-idling HepG2 cells).Transwell cells and scratches were used to detect their invasion and migration ability.The apoptosis was detected by flow cytometry.Results There was no significant difference between miR 26a NC group and HCC group in the number of invasion,migration rate and apoptosis rate of HepG 2 cells(P>0.05).Compared with miR 26a NC group,the invasion number,migration rate and apoptosis rate of HepG 2 cells in miR 26a group were significantly different from those in miR 26a NC group(P<0.05).There were significant differences in the levels ofβcatenin and Wnt 3 between miR 26a NC group and hepatoma group(P>0.05).There was no significant difference in the levels ofβcatenin and Wnt 3 between miR 26a NC group and miR 26a NC group(P<0.05).There was no significant difference in the mRNA levels ofβcatenin and Wnt 3 between miR 26a NC group and hepatoma group(P>0.05).The mRNA levels ofβcatenin and Wnt 3 in HepG2 cells of miR 26a group were significantly higher than those in miR 26a NC group(P<0.05).Conclusion Transfection of miR-26a mimics in liver cancer cells can inhibit cancer cell migration and invasion,accelerate apoptosis.The mechanism may be related to the inhibition ofβ-catenin and Wnt3 levels.

关 键 词：肝癌 miR-26a HEPG2细胞 迁移 Β-CATENIN Wnt3 

分 类 号：R735.7[医药卫生—肿瘤]