miR-122通过AXLHIF-1α通路抑制子宫内膜癌细胞增殖和上皮间质转化  

MiR-122 inhibits endometrial cancer cell proliferation and epithelial stromal transformation through AXL/HIF-1α pathway

作　　者：马香莲 冯海霞[1] 吴德慧[1] MA Xianglian;FENG Haixia;WU Dehui(Department of Gynecology,Qinghai Red Cross Hospital,Xining 810000,China)

机构地区：[1]青海红十字医院妇科,青海西宁810000

出　　处：《西部医学》2020年第9期1318-1323,共6页Medical Journal of West China

摘　　要：目的探讨miR-122对子宫内膜癌细胞(RL-952)增殖、上皮间质转化(EMT)及受体酪氨酸激酶(AXL)/缺氧诱导因-1α(HIF-1α)信号通路的影响。方法体外培养人子宫内膜癌细胞株RL-952,将细胞分为空白对照组(仅用培养基)、阴性对照组(转染miR-122 NC)、miR-122 mimics组(转染miR-122 mimics),采用实时荧光定量PCR(qRT-PCR)检测各组细胞miR-122水平,利用人胆囊收缩素/缩胆囊素八肽(CCK-8)试剂盒检测细胞增殖,流式细胞仪检测细胞凋亡,Transwell实验检测细胞迁移和侵袭能力,蛋白印迹(WB)法检测AXL、HIF-1α蛋白以及EMT相关蛋白E-cadherin、α-catenin、N-cadherin和Vimentin的表达变化。结果与空白对照组、阴性对照组相比,miR-122 mimics组RL-952细胞转染后48小时细胞增殖能力、细胞迁移数、细胞侵袭数、AXL、HIF-1α、N-cadherin和Vimentin蛋白表达均显著下降(P<0.05),E-cadherin、α-catenin蛋白表达、细胞凋亡率均明显升高(P<0.05),空白对照组与阴性对照组比较,细胞增殖能力、细胞迁移数、细胞侵袭数、细胞凋亡率、细胞AXL、HIF-1α、E-cadherin、α-catenin、N-cadherin和Vimentin蛋白表达差异均无统计学意义(P>0.05)。结论过表达miR-122可抑制子宫内膜癌细胞增殖、迁移、侵袭及上皮间质转化,促进细胞凋亡,可能是通过抑制AXL/HIF-1α通路而实现。Objective To study the effects of miR-122 on the proliferation,epithelial mesenchymal transition(EMT)and receptor tyrosine kinase(AXL)/hypoxia-inducible factor-1α(HIF-1α)signaling pathway of endometrial carcinoma cell line(RL-952).Methods Human endometrial carcinoma cell line RL-952 was cultured in vitro,and the cells were divided into blank control group(culture medium only),negative control group(miR-122 NC transfection)and miR-122 mimics group(miR-122 mimics transfection).The level of miR-122 was detected by real-time fluorescent quantitative PCR(qRT-PCR).The cell proliferation was detected by human cholecystokinin/cholecystokinin octapeptide(CCK-8)kit.The apoptosis was detected by flow cytometry.Transwell assay was used to detect cell migration and invasion.The expressions of AXL,HIF-1α,E-cadherin,α-Catenin,N-cadherin and vimentin proteins were detected by Western blot.Results Compared with blank control group and negative control group,the cell proliferation,cell migration,cell invasion,the expressions of AXL,HIF-1α,N-cadherin and vimentin proteins in RL-952 cells in miR-122 mimics group showed a significant decrease after 48h of transfection(P<0.05),the expressions of E-cadherin,α-Catenin proteins and apoptosis rate were significantly increased(P<0.05).There was no significant difference in cell proliferation,cell migration,cell invasion,apoptosis,expressions of AXL,HIF-1α,E-cadherin,α-Catenin,N-cadherin and Vimentin between the blank control group and the negative control group(P>0.05).Conclusion Over-expression of miR-122 can inhibit the proliferation,migration,invasion and epithelial stromal transformation of endometrial cancer cells,and promote cell apoptosis,which may be achieved by inhibiting the AXL/HIF-1αpathway.

关 键 词：MIR-122 子宫内膜癌细胞 受体酪氨酸激酶 缺氧诱导因-1α 增殖 上皮间质转化 

分 类 号：R737.33[医药卫生—肿瘤]