ApoA5基因-1131T>C多态性与肥胖患者血脂及载脂蛋白的相关性  

Association study between apolipoprotein A5 gene 1131T>C polymorphism and serum lipid and apolipoprotein levels in obese subjects

作　　者：白汝强 刘宇[2] 赵翀 高锦航[3] 刘瑞[3] BAI Ruqiang;LIU Yu;ZHAO Chong;GAO Jinhang;LIU Rui(Department of Stomatology,West China College of Stomatology,Sichuan University,Chengdu 610041,China;Department of Biochemistry and Molecular Biology,West China School of Preclinical and Forensic Medicine,Sichuan University,Chengdu 610041,China;Division of Digestive Diseases,West China Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川大学华西口腔医学院,四川成都610041 [2]四川大学华西基础与法医学院,四川成都610041 [3]四川大学华西医院消化疾病研究室,四川成都610041

出　　处：《西部医学》2020年第9期1376-1380,共5页Medical Journal of West China

基　　金：国家自然科学基金(39770322)。

摘　　要：目的探讨载脂蛋白A5(ApoA5)基因-1131T>C多态性与成都地区汉族人群肥胖及其血脂和载脂蛋白水平的关系。方法收集四川大学和四川师范大学校医院体检的教职工,均为无亲缘关系的成都地区汉族人295例,根据体重指数(BMI)分为肥胖组(BMI≥25kg/m2,102例)和对照组(BMI<25kg/m2,193例)。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析法,对两组ApoA5基因-1131T>C多态性进行了分析。酶法测定血清总TC、TG及高密度脂蛋白胆固醇(HDL-C)水平,免疫扩散法分析载脂蛋白的水平。结果ApoA5基因-1131T>C多态位点T、C等位基因频率在肥胖组和对照组分别为0.676、0.324和0.687、0.313,基因型频率分布符合Hardy-Weinberg平衡定律。ApoA5基因-1131T>C位点基因型频率和等位基因频率在肥胖组和对照组间比较差异无统计学意义(P>0.05)。对照组ApoA5基因-1131T/C位点CC基因型携带者的血清TG水平和TG/HDL-C比值均高于TT和TC基因型携带者,而HDL-C水平则低于后两种基因型携带者(P<0.05)。肥胖组CC基因型携带者的血清TG水平、TG/HDL-C比值均高于TT基因型携带者,LDL-C水平低于TT基因型携带者(P<0.05)。对照组CC基因型携带者血清载脂蛋白C-Ⅱ、C-Ⅲ和E水平均高于TT和TC基因型携带者(P<0.05);肥胖组CC基因型携带者血清apoB100水平低于TT和TC基因型携带者(P<0.05)。结论ApoA5基因-1131T>C多态性与肥胖的发生无相关性。ApoA5基因-1131T>C多态性与非肥胖及肥胖患者的血清TG水平和TG/HDL-C比值增高有关,且与部分血清载脂蛋白水平的改变存在关联。Objective To investigate effects of apolipoprotein A5 apoA5)gene-1131T>C polymorphism on serum lipid and apolipoprotein levels in obese subjects from a population of Chinese Han nationality in Chengdu.Methods 102 obese patients and 193 non-obese subjects were studied using the polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Triglyceride(TG),total cholesterol(TC)and high density lipoprotein cholesterol(HDL-C)were measured by enzymatic kits and apolipoproteins AI,AII,B100,CII,CIII and E were measured by the RID kits.Results The allele frequencies of T and C were 0.676 and 0.324 in obese group,and 0.687 and 0.313 in control group,respectively.There were no significant differences of genotype and allele frequencies between the two groups(P>0.05).In control group,subjects with CC genotype was associated with significantly higher serum triglycerides,levels and TG/HDL-C ratio and lower HDL-C levels,when compared with those with the TT or TC genotypes.In obese group,subjects with CC genotype was associated with higher TG and TG/HDL-C,and lower low density lipoprotein cholesterol(LDL-C)level when compared with those with genotype TT(P<0.05).In addition,in non-obese group subjects with CC genotype showed higher apoC-Ⅱ、C-Ⅲand E levels when compared to those with TT or CC genotype.In obese group subject with CC genotype showed lower apoB levels,when compared to those with TT or TC genotype.Conclusion There was no correlation between APOA5 gene 1131T>C polymorphism and obesity.The polymorphism of APOA5 gene 1131T>C is related to the increase of serum TG level and TG/HDL C ratio in non obese and obese patients,and is related to the change of some serum apolipoproteins.

关 键 词：肥胖 载脂蛋白A5 血脂 载脂蛋白 遗传多态性 

分 类 号：R394.5[医药卫生—医学遗传学]