微塑料与多环芳烃菲复合胁迫对七彩神仙鱼稳定同位素和生态化学计量学特征的影响  

Effects of microplastics and phenanthrene on the stable isotope and ecological stoichiometry of juvenile discus fish(Symphysodon haraldi)

作　　者：冯晓飒 温彬 刘君恒 张源[1,2,3,4] 高建忠[1,2,3,4] 陈再忠[1,2,3,4] FENG Xiaosa;WEN Bin;LIU Junheng;ZHANG Yuan;GAO Jianzhong;CHEN Zaizhong(National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China;Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学,水产科学国家级实验教学示范中心,上海201306 [2]上海海洋大学,农业农村部淡水水产种质资源重点实验室,上海201306 [3]上海海洋大学,上海水产养殖工程技术研究中心,上海201306 [4]上海海洋大学,水产动物遗传育种中心上海市协同创新中心,上海201306

出　　处：《水产学报》2020年第10期1742-1751,共10页Journal of Fisheries of China

基　　金：上海市扬帆计划(19YF1419400);国家自然科学基金(31902376)。

摘　　要：为探讨微塑料和多环芳烃菲对七彩神仙鱼生长、能量存储、稳定同位素及生态化学计量学特征的影响,实验设置了3个微塑料浓度(0、100、1000μg/L)和2个菲水平(0、50μg/L),共3×2个暴露组,进行8周的养殖实验。结果显示:①幼鱼末体质量未受微塑料和菲影响,但菲会降低肥满度,且二者对肝体指数有交互影响:50μg/L菲下100μg/L微塑料增加肝体指数,100μg/L微塑料下菲可提高肝体指数。②幼鱼蛋白质含量随菲浓度升高而增加,粗脂肪和碳水化合物均受微塑料和菲交互影响:0μg/L菲下微塑料增加会提高粗脂肪含量,但会降低碳水化合物含量;1000μg/L微塑料下菲增加会降低粗脂肪含量,但会提高碳水化合物含量。③幼鱼δ13C随微塑料增加而降低,δ15N受二者交互影响,但不同条件下无显著差异。④微塑料会增加幼鱼C含量,菲则增加N含量,而P含量还受二者交互影响,0μg/L菲下100μg/L微塑料降低P含量,而50μg/L菲下1000μg/L微塑料降低P含量;100μg/L微塑料下菲增加会提高P含量,但1000μg/L微塑料下菲增加会降低P含量;幼鱼C/N受微塑料和菲单一暴露影响,而N/P和C/P还受二者交互影响。研究表明,微塑料和菲复合胁迫对七彩神仙鱼幼鱼生长和存活未造成显著影响,但可能会通过改变鱼体组织能量存储,导致其稳定同位素和生态化学计量学特征发生变化。To investigate the effects of microplastics and phenanthrene on the growth,energy storage,stable isotope composition and ecological stoichiometry of the juvenile discus fish(Symphysodon haraldi),we set three concentrations of microplastics(0,100 and 1000μg/L)and two levels of phenanthrene(0 and 50μg/L),forming a total of six exposure combinations for eight weeks.The results showed that the final body weight of fish was not influenced by exposure to microplastics and phenanthrene,but exposure to phenanthrene reduced the condition factor.The hepatosomatic index(HSI)was influenced by the interaction of microplastics and phenanthrene.Exposure to 100μg/L microplastics increased HSI under 50μg/L phenanthrene,and exposure to phenanthrene increased HSI under 100μg/L microplastics.The protein content increased with increased concentrations of phenanthrene.Microplastics and phenanthrene had an interaction effect on carbohydrate and crude fat contents.Increased concentrations of microplastics increased crude fat content but reduced carbohydrate level under 0μg/L phenanthrene,and exposure to phenanthrene decreased crude fat content but increased carbohydrate level under 1000μg/L microplastics.Theδ13C of juveniles decreased with increase concentrations of microplastics.Theδ15N was influenced by the interaction of microplastics with phenanthrene,but no significant differences between exposure combinations were observed.Exposure to microplastics increased body C content and exposure to phenanthrene increased N level.Moreover,microplastics and phenanthrene had an interaction effect on P content.Exposure to 100μg/L microplastics reduced P content under 0μg/L phenanthrene but exposure to 1000μg/L microplastics reduced P level under 50μg/L phenanthrene.Exposure to phenanthrene increased P content under 100μg/L microplastics but reduced P level under 1000μg/L microplastics.The C/N ratio was influenced by exposure to microplastics or phenanthrene alone,but ratios of N/P and C/P were also affected by the interaction of

关 键 词：七彩神仙鱼 微塑料 菲 稳定同位素 生态化学计量学 

分 类 号：X171[环境科学与工程—环境科学] S917[农业科学—水产科学]