节瓜ACC合酶基因CqACS的克隆与表达分析  

Analysis on Cloning and Expression of ACC Synthase(CqACS)Gene from Benincasa hispida

作　　者：彭琴 王敏 彭庆务[1] 江彪[1,3] 林毓娥 刘文睿[1,3] 梁肇均[1] 李玲 何晓明[1,3] PENG Qin;WANG Min;PENG Qing-wu;JIANG Biao;LIN Yu-e;LIU Wen-rui;LIANG Zhao-jun;LI Ling;HE Xiao-ming(Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,Guangdong,China;School of Life Sciences,South China Normal University,Guangzhou 510631,Guangdong,China;Guangdong Key Laboratory for New Technology Research of Vegetables,Guangzhou 510640,Guangdong,China)

机构地区：[1]广东省农业科学院蔬菜研究所,广东广州510640 [2]华南师范大学生命科学学院,广东广州510631 [3]广东省蔬菜新技术研究重点实验室,广东广州510640

出　　处：《中国蔬菜》2020年第10期35-41,共7页China Vegetables

基　　金：广东省科技计划项目(2016B020201005);广州市农业产业发展专项资金(1710023);广东省重点领域研发计划项目(2018B020202007)。

摘　　要：为分析ACC合酶与节瓜雌性之间的关系,以雌性自交系(A36)和普通雌雄同株自交系(SX)为材料,克隆得到节瓜ACC合酶(CqACS)基因,并与葫芦科中已知的ACC合酶基因进行同源序列比对分析、构建进化树;对A36幼苗叶片进行赤霉素(GA3)处理后用qRT-PCR对CqACS基因进行表达分析,并统计赤霉素处理前后20节内的雌花率。结果表明,CqACS基因在雌性自交系A36中的长度为1318 bp,普通雌雄同株自交系SX中长度为1637 bp。经对比分析,SX比A36多了91、97、117 bp 3个片段,除此之外只有2个碱基的差别。同源序列比对发现,A36中的CqACS基因与西瓜中的Cit-ACS1基因同源性最高,为95.31%,与黄瓜中的Cs-ACS1基因同源性为91.13%;SX中的CqACS基因与西瓜中Cit-ACS1基因同源性为78.04%,与黄瓜中的Cs-ACS1基因同源性为76.06%。GA3处理后,A36的平均雌花节率(20节位内)为45%,而对照为90%;qRT-PCR分析发现,与对照相比,CqACS基因在GA3处理后的相对表达量显著上调,且在第3次处理后4 h上调最大。In order to find out the relation between ACC synthase and female chieh-qua,this study took female inbred line(A36)and normal monoacious inbred line(SX)as material,gained CqACS gene through coloning,then conducted homologous sequence comparative analysis with the known CqACS gene;and constructed evolutionary tree.The expression profile of CqACS in seedling leaf blades of A36 was analyzed after treatment with gibberellin(GA3)by qRT-PCR on CqACS gene.The female flower rate within 20 nodes was carried out statistic analysis before and after gibberellin treatment.The results showed that the length of CqACS gene in A36 of female inbred line was 1318 bp and that in normal monoecism selfing line in SX was 1637 bp.After comparative analysis,we found that SX in 3 bp base element:91,97 bp and 117 bp were more than detected in A36,and only 2 basic group differences were found between them.The homologous sequence comparison revealed that CqACS gene in A36 had the highest homology with Cit-ACS1 gene in watermelon,reaching 95.31%.And the homology with cucumber Cs-ACS1 was 91.31%.CqACS gene in SX had the homology with Cit-ACS1 gene in watermelon,reaching 78.04%,and the homology with cucumber Cs-ACS1 was 76.06%.After GA3 treatment,the average female flower rate(within 20th node order)of GA3 treatment group in A36 was 45%.While,that of the contrast group was 90%.qRT-PCR analysis discovered that the expression quantity of CqACS gene after GA3 treatment was significantly up-regulated.Compared with the contrast group.Moreover,4 h after the third time treatment the up-regulated range was the largest.

关 键 词：节瓜 ACC合成酶 CqACS克隆 表达分析 

分 类 号：S642.9[农业科学—蔬菜学]