利川产区显齿蛇葡萄嫩叶总黄酮成分和抗菌活性研究  

Study on compositions and antibacterial activities of the total flavonoids from the tender leaves of Ampelopsis grossedentata(Hand.-Mazz.)W.T.Wang in Lichuan

作　　者：李宇 刘翠君[1] 廖璐婧[1] 郭汉玖[1] 冉亚兰 刘丹 罗建群 何美军[1] LI Yu;LIU Cuijun;LIAO Lujin;GUO Hanjiu;RAN Yalan;LIU Dan;LUO Jianqun;HE Meijun(Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi, Hubei 445000, China;Agricultural and Rural Bureau of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei 445000, China;Medical School, Hubei Minzu University, Enshi, Hubei 445000, China;Enshi Selenium Commander and Ecological Agriculture Company, Enshi, Hubei 445000, China)

机构地区：[1]湖北省农业科学院中药材研究所,湖北恩施445000 [2]恩施土家族苗族自治州农业农村局,湖北恩施445000 [3]湖北民族大学医学部,湖北恩施445000 [4]恩施硒司令生态农业科技发展有限公司,湖北恩施445000

出　　处：《华中师范大学学报（自然科学版）》2020年第5期820-825,共6页Journal of Central China Normal University：Natural Sciences

基　　金：湖北省农业科学院中药材研究所2019年青年基金项目(2019ZYCJJ05)。

摘　　要：采用超声波辅助乙醇溶液提取利川产区显齿蛇葡萄嫩叶总黄酮,通过液质联用高分辨电喷雾质谱(LC-HR-ESIMS)技术鉴定其组分,采用高效液相外标法测定各组分含量,运用滤纸片法测试显齿蛇葡萄嫩叶总黄酮对10株供试菌的体外抑菌活性.结果表明:从显齿蛇葡萄嫩叶总黄酮中鉴定和定量了7个黄酮组分:二氢杨梅素(18.57%)、杨梅苷(0.53%)、杨梅素(1.29%)、花旗松素(1.38%)、槲皮素(0.51%)、三叶豆苷(0.78%)、山柰酚(0.45%);显齿蛇葡萄嫩叶总黄酮对苏云金芽孢杆菌、枯草芽孢杆菌、耐甲氧西林金黄色葡萄球菌、溶藻弧菌有中度敏感抑制活性,其最小抑菌浓度(MIC)值为1.0 mg·mL^-1、2.0 mg·mL^-1、2.0 mg·mL^-1、1.0 mg·mL^-1.The total flavonoids from the tender leaves of Ampelopsis grossedentata(vine tea)were extracted by ultrasonic.The total flavonoids were identified by LC-HR-ESIMS,and the content of each component was determined by external standard method.The antibacterial activity of total flavonoids against 10 clinical drug-resistant bacterias in vitro by filter paper method.The results showed that 7 flavonoids were identified from the total flavonoids in the tender leaves.The main components of total flavonoids were dihydromyricetin(18.57%),myricitrin(0.53%),myricetin(1.29%),taxifolin(1.38%),trifolin(0.78%),kaempferol(0.45%),and quercetin(0.51%).The total flavonoids of vine tea had medium inhibitory activity against bacillus thuringiensis,methicillin-resistant staphylococcus aureus,bacillus subtilis and vibrio alginolyticus XSBZ14,with the minimum inhibitory concentration(MIC)value of 1.0 mg·mL^-1,2.0 mg·mL^-1,2.0 mg·mL^-1 and 1.0 mg·mL^-1,respectively.

关 键 词：显齿蛇葡萄嫩叶 抗菌活性 总黄酮 最小抑菌浓度 

分 类 号：Q939.13[生物学—微生物学] R914.4[医药卫生—药物化学]