Propentofylline对中性粒细胞的调节作用  

The Effects of Propentofylline on Human Neutrophils

作　　者：张煜[1] 巴图[1] 

机构地区：[1]白求恩医科大学,长春130021

出　　处：《中国临床药理学杂志》2000年第1期55-57,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：本研究探讨 Ｐｒｏｐｅｎｔｏｆｙｌｌｉｎｅ对中性粒细胞的调节作用及其它的作用机制。采用鲁米诺依赖的全血化学发光法测定ｆＭＬＰ激活的中性粒细胞产生过氧化氢。结果显示Ｐｒｏｐｅｎｔｏｆｙｌｌｉｎｅ以浓度依赖形式抑制中性粒细胞产生过氧化氢，并增强腺苷对中性粒细胞的抑制作用，但不影响ＮＥＣＡ对中性粒细胞的抑制作用。结果提示，Ｐｒｏｐｅｎｔｏｆｙｉｌｉｎｅ通过抑制细胞对腺苷的摄取，增加局部腺苷水平，增强腺苷对中性粒细胞的抑制作用。这种作用也可能是Ｐｒｏｐｅｎｔｏｆｙｌｌｉｎｅ保护心脑组织损伤的机制之一。In this study, we tested the effects and mechanisms of propentofylline on human neutrophils. The neutrophils were stimulated by fMLP (formyl-MetLeuPhe) and hydrogen peroxide was determined by the luminol amplified chemiluminescence. Results showned that propentofylline inhibited the hydrogen peroxide production by neutrophils in concentration-dependent manner The inhibitory effect of adenosinc on fMLP-stimulated neutrophil hydrogen peroxide was markedly increased in the presence of propentofylline. But that of NECA (5 -N- ethylcarboxamidoadenosine) was unaffected, indicated that the inhibitory effect of propentofylline on neutrophils was mediated by inhibiting adenosine uptake and increasing the local concentration of adenosine. This effect could be a mechanism of the protective effects of propentofylline on the ischemia of brain and heart.

关 键 词：中性粒细胞 腺苷 PROPENTOFYLLINE 过氧化氢 全血化学发光法 鲁米诺反应 组织缺血 

分 类 号：R364.12[医药卫生—病理学] R965[医药卫生—基础医学]