背根神经节核苷酸寡聚化域样受体2在大鼠神经病理痛中的作用  

Role of Nod-like receptor family pyrin domain-containing 2 in dorsal root ganglion in neuropathic pain in rats

作　　者：李黎[1] 沈江[1] 徐青荣 高操[1] 何晓峰[1] 张如锦[1] Li Li;Shen Jiang;Xu Qingrong;Gao Cao;He Xiaofeng;Zhang Rujin(Department of Anesthesiology,First People′s Hospital of Changzhou(the Third Affiliated Hospital of Soochow University),Changzhou 213000,China)

机构地区：[1]江苏省常州市第一人民医院(苏州大学附属第三医院)麻醉科,213000

出　　处：《中华麻醉学杂志》2020年第5期606-609,共4页Chinese Journal of Anesthesiology

基　　金：常州市卫生计生委指导性科技项目(WZ201810);常州市卫健委青年人才科技项目(QN201925)。

摘　　要：目的评价背根神经节核苷酸寡聚化域样受体2(NLRP2)在大鼠神经病理性痛中的作用。方法取鞘内置管成功的雄性SD大鼠32只,体重200~250 g,2~3月龄,采用随机数字表法分为4组(n=8):假手术组(S组)、神经病理性痛组(NP组)、神经病理性痛+NLRP2-siRNA组(NP+siRNA组)和神经病理性痛+NLRP2-scrRNA组(NP+scrRNA组)。S组仅暴露右侧坐骨神经不结扎;NP组、NP+siRNA组和NP+scrRNA组采用慢性坐骨神经缩窄性损伤术制备大鼠神经病理性痛模型;NP+siRNA组和NP+scrRNA组于术前3 d时分别鞘内注射NLRP2-siRNA和NLRP2-scrRNA。于术前1 d(T0)、术后1、3、7和10 d(T1-4)时测定机械缩足反应阈(MWT),最后1次测定痛阈后处死大鼠,取术侧L4,5节段背根神经节,采用Western blot法检测NLRP2和caspase-1表达,采用RT-PCR法检测NLRP2 mRNA表达,采用ELISA法测定IL-1β含量。结果与T0时比较,NP组、NP+siRNA组和NP+scrRNA组T2-4时MWT降低(P<0.05);与S组比较,NP组T2-4时MWT降低,背根神经节NLRP2及其mRNA表达和caspase-1表达上调,IL-1β含量升高(P<0.05);与NP组比较,NP+siRNA组T2-4时MWT升高,背根神经节NLRP2及其mRNA表达和caspase-1表达下调,IL-1β含量减少(P<0.05),NP+scrRNA组各时点上述指标差异无统计学意义(P>0.05)。结论背根神经节NLRP2参与了大鼠神经病理性痛发生的过程,机制与形成NLRP2炎症小体诱发外周神经炎症有关。Objective To evaluate the role of Nod-like receptor family pyrin domain-containing 2(NLRP2)in the dorsal root ganglion(DRG)in neuropathic pain(NP)in rats.Methods Thirty-two male Sprague-Dawley rats in which intrathecal catheters were successfully implanted,aged 2-3 months,weighing 200-250 g,were divided into 4 groups(n=8 each)using a random number table method:sham operation group(S group),NP group,NP plus NLRP2-siRNA group(NP+siRNA group)and NP plus NLRP2-scrRNA group(NP+scrRNA group).The right sciatic nerve was only exposed but not ligated in group S.NP was induced by chronic constriction injury(CCI)to the sciatic nerve in anesthetized rats in group NP,group NP+siRNA and group NP+scrRNA.NLRP2-siRNA and NLRP2-scrRNA were intrathecally injected at 3 days before CCI in group NP+siRNA and group NP+scrRNA,respectively.The mechanical paw withdrawal threshold(MWT)was measured at 1 day before CCI(T0)and 1,3,7 and 10 days after CCI(T1-4).The rats were sacrificed after the last measurement of pain threshold,and the L4,5 segments of the DRG on the operated side were removed for determination of the expression of NLRP2 and caspase-1(by Western blot),the expression of NLRP2 mRNA(by real-time polymerase chain reaction)and interleukin-1beta(IL-1β)content(by enzyme-linked immunosorbent assay).Results The MWT was significantly lower at T2-4 than at T0 in group NP,group NP+siRNA and group NP+scrRNA(P<0.05).Compared with group S,the MWT was significantly decreased at T2-4,the expression of NLRP2 protein and mRNA and caspase-1 was up-regulated,and the content of IL-1βwas increased in group NP(P<0.05).Compared with group NP,the MWT was significantly increased at T2-4,the expression of NLRP2 protein and mRNA and caspase-1 was down-regulated,and the content IL-1βwas decreased in group NP+siRNA(P<0.05),and no significant change was found in the parameters mentioned above in group NP+scrRNA(P>0.05).Conclusion NLRP2 in DRG is involved in the development of NP,and the mechanism is related to NLPR2 inflammasomes-induced peripheral neur

关 键 词：背根神经节 神经痛 核苷酸寡聚化域样受体2 

分 类 号：R741.02[医药卫生—神经病学与精神病学]