线叶龙胆(Gentiana lawrencei var.farreri)ITS序列杂合性与遗传分化分析  

Analysis of ITS heterozygosity and genetic divergence in Gentiana lawrencei var.farreri

作　　者：付鹏程 林清银 孙姗姗 FU Peng-cheng;LIN Qing-yin;SUN Shan-shan(School of Life Science,Luoyang Normal University,Luoyang 471934,China)

机构地区：[1]洛阳师范学院生命科学学院,洛阳471934

出　　处：《生物学杂志》2020年第5期44-47,共4页Journal of Biology

基　　金：国家自然科学基金(31600296)。

摘　　要：线叶龙胆(Gentiana lawrencei var.farreri)是龙胆属的常见种之一,分布于青藏高原地区,具有药用与观赏价值。ITS是重要的分子标记之一,可用于植物群体遗传与进化研究;然而由于其杂合性较高,为后续分析带来一定困难。以线叶龙胆7个居群共39个个体为对象,通过基因克隆与测序,共得到81条序列,鉴定出76个单倍型。结果表明ITS序列在线叶龙胆中存在很高的杂合性,居群遗传多样性高;且遗传变异的87.61%来自居群内,仅有12.39%来自于居群间。在鉴定的单倍型中,97.36%为单个个体所特有,个体间很少共享同一种单倍型;进一步的系统发育分析表明这些单倍型无法聚成高支持率的支系。研究揭示了线叶龙胆ITS序列存在高度杂合性,可为龙胆属的进化研究提供参考。Gentiana lawrencei var.farreri,which has medical and gardening value,is a common species in Gentiana and distributes on the Qinghai-Tibetan Plateau.Nuclear ribosomal internal transcribed spacer(ITS)is a common molecular marker used in phylogenetics and population genetics,but has difficulty in sequencing and data analysis due to heterozygosity.A total of 39 individuals from 7 populations of G.lawrencei var.farreri were sequenced after cloning in this study.The obtained 81 sequences identify 76 haplotypes in total.For the genetic variations,87.61%occured within population and only 12.39%occured among populations.Haplotypes,which were rarely shared among individuals(2.64%),did not show a clear phylogenetic relationship.In conclusion,this study showed high ITS heterozygosity in G.lawrencei var.farreri and would be helpful in researches about Gentiana evolution.

关 键 词：线叶龙胆 ITS 杂合性 遗传分化 

分 类 号：Q943[生物学—植物学]