长链非编码RNA HAGLROS对非小细胞肺癌A549细胞迁移和侵袭能力的影响  

Effects of Long Non-coding RNA HAGLROS on Migration and Invasion Ability of Non-Small Cell Lung Cancer A549 Cells

作　　者：杨雨晴 李洋洋 程旭 韩慧明[1] 李咏梅[1] 朱滕滕 YANG Yuqing;LI Yangyang;CHENG Xu;HAN Huiming;LI Yongmei;ZHU Tengteng(Basic Medical College of Beihua University,Jilin 132013,China;Hongqiao International Institute of Medicine,School of Medicine of Shanghai Jiao Tong University,Shanghai 200336,China)

机构地区：[1]北华大学基础医学院,吉林吉林132013 [2]上海交通大学医学院虹桥国际医学研究院,上海200336

出　　处：《北华大学学报（自然科学版）》2020年第5期608-612,共5页Journal of Beihua University（Natural Science）

基　　金：国家自然科学基金面上项目(81573231).

摘　　要：目的探讨长链非编码RNA HAGLROS(lncRNA HAGLROS)对非小细胞肺癌A549细胞系迁移和侵袭能力的影响.方法采用siRNA(小RNA干扰技术)对细胞进行转染;同时采用实时荧光定量PCR技术(qPCR)检测相关目的基因的表达水平;应用细胞划痕实验检测细胞的迁移能力;应用Transwell实验检测细胞的侵袭能力.结果qPCR结果显示:对A459细胞进行转染后,siRNA-lncRNA HAGLROS-homo-435敲减效率最高,差异具有统计学意义(P<0.05);划痕实验和Transwell实验结果表明:实验组与对照组比较,细胞迁移能力、细胞侵袭能力均下降,差异具有统计学意义(P<0.05).结论lncRNA HAGLROS在肺癌细胞系A549中作为促癌基因发挥作用,可为肺癌的诊断及治疗提供临床参考.Objective To investigate the effect of long non-coding RNA HAGLROS(lncRNA HAGLROS)on migration and invasion of non-small cell lung cancer A549 cell line.Method Cells were transfected with siRNA(small RNA interference technology);the expression level of related target genes was detected by real-time fluorescence quantitative PCR(qPCR);cell scratch test was used to detect cell migration ability;Transwell test was used to determine cell invasion ability.Results The qPCR results showed that siRNA-lncRNA HAGLROS-homo-435 had the highest knock-down efficiency after transfection of A459 cells(P<0.05).Scratch results and Transwell results showed that the migration ability and invasive ability of cells in the experimental group were decreased comparing with the control group,and the difference was statistically significant(P<0.05).Conclusion lncRNA HAGLROS is an oncogene in lung cancer cell line A549,which can provide clinical diagnosis and treatment for lung cancer.

关 键 词：非小细胞肺癌A549 lncRNA HAGLROS 细胞迁移 细胞侵袭 

分 类 号：R363.1[医药卫生—病理学] R734.2[医药卫生—基础医学]