miR-21在幽门螺杆菌诱导的胃癌发生发展中的作用  

The Role of miR-21 in the Development of Gastric Cancer Induced by Helicobacter Pylori

作　　者：何文婧[1] 刘静[1] 武静静 米思蓉[3] HE Wenjing;LIU Jing;WU Jingjing(Yulin First Hospital,Yulin,719000)

机构地区：[1]陕西省榆林市第一医院,719000 [2]陕西省靖边县人民医院,718500 [3]延安大学附属医院东关分院,716000

出　　处：《实用癌症杂志》2020年第10期1600-1603,共4页The Practical Journal of Cancer

基　　金：榆林市科学技术项目(编号:201904)。

摘　　要：目的探讨miR-21在幽门螺杆菌(helicobacter pylori,Hp)诱导的胃癌发生中的作用。方法选择120例胃癌患者(胃癌组)与120例胃溃疡患者(胃溃疡组),取2组患者的全血样本,采用qRT-PCR检测miR-21表达水平,调查患者的临床特征与随访患者的预后。结果胃癌组的miR-21相对表达水平与表达阳性率显著高于胃溃疡组(P<0.05)。在120例胃癌患者中,不同Dukes分期、淋巴结转移、分化程度、远处转移、浸润深度患者的miR-21表达阳性率对比差异有统计学意义(P<0.05)。胃癌组患者随访至今,平均随访时间为(15.22±2.48)月,死亡12例,死亡率为10.0%;单因素和多因素Cox回归分析显示miR-21表达阳性率、Dukes分期、淋巴结转移、分化程度、远处转移、浸润深度都为影响胃癌患者预后的危险因素(P<0.05)。结论miR-21在Hp诱导的胃癌患者全血中呈现高表达状况,与胃癌的临床特征显著相关,可以作为预测患者预后的潜在生物标志物。Objective To investigate the role of miR-21 in the development of Helicobacter pylori(Hp)induced by Helicobacter pylori.Methods 120 patients with gastric cancer(gastric cancer group)and 120 patients with gastric ulcer(gastric ulcer group)were selected as the study subjects.The whole blood samples of the 2 groups were collected and the expression level of miR-21 were detected by qRT-PCR,the clinical characteristics of the patients and the prognosis of the patients were investigated.Results The relative expression and expression of miR-21 in the gastric cancer group were significantly higher than those in the gastric ulcer group(P<0.05).In the 120 patients with gastric cancer,the positive rates of miR-21 expression in patients with different Dukes stage,lymph node metastasis,differentiation,distant metastasis,and invasive depth were statistically significant(P<0.05).The patients in the gastric cancer group were followed up for an average of 15.22±2.48 months,there were 12 patients were died,and the mortality rates were 10.0%.Univariate and multivariate Cox regression analysis showed miR-21 expression positive rate,Dukes stage,lymph node metastasis,differentiation degree,Distant metastasis and depth of invasion were risk factors for the prognosis of patients with gastric cancer(P<0.05).Conclusion miR-21 are highly expressed in Hp-induced whole blood of gastric cancer,and is significantly correlated with the clinical features of patients.It can be used as potential biomarker for predicting the prognosis of gastric cancer.

关 键 词：MIR-21 胃癌 幽门螺杆菌 生物标志物 

分 类 号：R735.2[医药卫生—肿瘤]