别隐品碱和原阿片碱在猪肝S9的代谢研究  

Metabolism of Allocryptopine and Protopine in S9 of Pig Liver

作　　者：赵雪娇 黄亚军 张明军 刘兆颖 ZHAO Xue-jiao;HUANG Ya-jun;ZHANG Ming-jun;LIU Zhao-ying(College of Veterinary Medicine,Hunan Agricultural University,Hunan Engineering Research Center of Veterinary Drugs,Changsha 410128,China)

机构地区：[1]湖南农业大学动物医学院,湖南省兽药工程技术研究中心,长沙410128

出　　处：《中国兽药杂志》2020年第7期41-47,共6页Chinese Journal of Veterinary Drug

基　　金：国家重点研发计划项目(2017YFD0501503)。

摘　　要：试验采用高效液相色谱/四极杆飞行时间质谱(HPLC-QqTOF-MS)技术,进行别隐品碱(ALL)和原阿片碱(PRO)在猪肝S9中的代谢研究。通过比较这些代谢物的精确分子量和产物离子与前体离子或代谢物的变化,进行了这些代谢物的结构鉴定。结果显示,在猪肝S9中检测到低浓度的ALL和PRO,分别鉴定出4种ALL和3种PRO的代谢物,O-去甲基和C2-C3位亚甲二氧环环裂后去甲基分别为ALL和PRO是猪肝体外的主要代谢途径,且2,3-亚甲二氧环去甲基比9,10-亚甲二氧环去甲基更占优势。其研究结果可为解释博普总碱的药动学和食品安全性评价提供理论依据。High performance liquid chromatography/quadrupole-time-of-flight(HPLC-QqTOF-MS)mass spectrometry method was used to study the metabolism of allocryptopine(ALL)and protopine(PRO)in pig liver S9.The structure of these metabolites was performed by comparing the exact molecular weights of these metabolites and the changes of productions with precursorions or metabolites.The results showed that 4 ALL metabolites and 3 PRO metabolites were detected in S9 of pig liver.It can be concluded that demethylation at O-demethylation and demethylation at C2-C3 were the main metabolic path ways of ALL and PRO,respectively.Moreover,2,3-methoxycyclodemethylation of All and PRO is more advantageous than 9,10-methoxycyclodemethylation.The results can provide theoretical basis for the pharmacokinetic and food safety evaluation of Plume Poppy total alkaloid.

关 键 词：HPLC-QqTOF-MS 别隐品碱 原阿片碱 猪肝 代谢 

分 类 号：S853.7[农业科学—临床兽医学]