荷叶离褶伞子实体化学成分  

Chemical constituents from fruiting bodies of Lyophyllum decastes

作　　者：郑中庸 韩俊杰[1,2] 米鹏程 陈保送 宝丽 席亚丽[3] 王政迪 谢丽萍 郭长彬 魏生龙 刘宏伟[1,2] ZHENG Zhong-Yong;HAN Jun-Jie;Ml Peng-Cheng;CHEN Bao-Song;BAO Li;XI Ya-Li;WANG Zheng-Di;XIE Li-Ping;GUO Chang-Bin;WEI Sheng-Long;LIU Hong-Wei(State Key Laboratory of Mycology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;Savaid Medical School,University of Chinese Academy of Sciences,Beijing 100049,China;Gansu Engineering Laboratory of Applied Mycology,Hexi University,Zhangye,Gansu 730400,China;Department of Chemistry,Capital Normal University,Beijing 10004&China)

机构地区：[1]中国科学院微生物研究所真菌学国家重点实验室,北京100101 [2]中国科学院大学存济医学院,北京100049 [3]河西学院甘肃省应用真菌工程实验室,甘肃张掖730400 [4]首都师范大学化学系,北京100048

出　　处：《菌物学报》2020年第9期1774-1782,共9页Mycosystema

基　　金：重点生态保护修复治理专项资金(张山水湖办[2018]20号);甘肃省引导科技创新发展专项资金(2017zx-10);国家自然科学基金(21472233);国家重点研发计划(2018YFD0400203)。

摘　　要：本文对祁连山野生荷叶离褶伞Lyophyllumdecastes子实体的化学成分和生物活性进行研究。采用硅胶色谱、高效液相色谱等多种方法进行分离纯化得到8个化合物,通过MS、NMR和电子圆二色谱(ECD)等方法确定了化学结构,其中有4个为聚炔类化合物。化合物1作为天然产物系首次报道,其相绝对构型是通过比较ECD的方法确定。对所得聚炔类化合物应用细胞模型进行抗氧化活性(CAA)指标检测,化合物1–4均呈现一定抗氧化活性,其中化合物1的抗氧化活性最强,其EC50为(24.73±6.12)μmol/L。聚炔类化合物1–4为荷叶离褶伞首次报道成分,可作为祁连山野生荷叶离褶伞HPLC-DAD化学表征参考化合物。The chemical constituents and biological activities of the fruiting bodies of Lyophyllum decastes were studied.Fractionation of the crude extract by the modern chromatographic separation techniques afforded eight secondary metabolites,four of which were polyacetylene.The structures of those compounds were elucidated by nuclear magnetic resonance(NMR),mass spectrometry(MS)techniques as well as circular dichroism spectral(CD)and electron circular dichroism(ECD).The antioxidant activities of these isolated compounds were evaluated using cellular antioxidant activity(CAA)assay.Compounds 1–4 all showed antioxidant activity to a certain extent,of which compound 1 showed the strongest activity with EC50(24.73±6.12)μmol/L.Polyacetylene compounds 1–4 were isolated from L.decastes for the first time,providing reference compounds for further HPLC-DAD analysis.

关 键 词：荷叶离褶伞 次级代谢产物 聚炔 细胞内抗氧化 

分 类 号：R284.1[医药卫生—中药学]