MYCT1通过PRSS21抑制喉癌细胞的增殖和迁移  

MYCT1 inhibits the proliferation and migration of laryngeal carcinoma cells via PRSS21

作　　者：陈芸 孙媛媛[1] 富伟能[1] CHEN Yun;SUN Yuanyuan;FU Weineng(Department of Medical Genetics,School of Life Science,China Medical University,Shenyang 110122,China)

机构地区：[1]中国医科大学生命科学学院医学遗传学教研室,沈阳110122

出　　处：《中国医科大学学报》2020年第10期876-881,共6页Journal of China Medical University

基　　金：国家自然科学基金(81372876);辽宁省重点研发计划(2017225021);辽宁省高等学校基本科研项目(LQNK201726)。

摘　　要：目的探讨MYCT1通过PRSS21对喉癌细胞增殖和迁移的影响。方法应用实时PCR和Western blotting方法检测喉癌中PRSS21的表达水平及MYCT1对PRSS21表达水平的影响;应用CCK-8实验和克隆形成实验检测MYCT1通过PRSS21对喉癌细胞增殖和克隆形成能力的影响;应用划痕实验检测MYCT1通过PRSS21对喉癌细胞迁移能力的影响。结果PRSS21在喉癌中高表达(P<0.05);与对照组相比,MYCT1显著降低PRSS21在喉癌细胞中mRNA和蛋白质的表达水平(P<0.05);MYCT1通过PRSS21显著抑制喉癌细胞的增殖和克隆形成(P<0.05);MYCT1通过PRSS21显著抑制喉癌细胞的迁移能力(P<0.05)。结论MYCT1可通过抑制PRSS21的表达抑制喉癌细胞的增殖和迁移。Objective To investigate the effects of MYCT1 on the PRSS21-mediated proliferation and migration of laryngeal carcinoma cells.Methods Real-time PCR and Western blotting were used to detect PRSS21 mRNA and protein expression,respectively in the context of MYCT1 silencing or overexpression in laryngeal carcinoma.The CCK-8 and clone formation assays were applied to monitor the effects of MYCT1 on the PRSS21-mediated proliferation and survival of laryngeal cancer cells.A scratch assay was further conducted to monitor the effect of MYCT1 on the migration(PRSS21-mediated)of laryngeal cancer cells.Results PRSS21 was highly expressed in laryngeal carcinoma(P<0.05).Compared to controls,MYCT1 significantly inhibited the expression of PRSS21 both at mRNA and protein levels in laryngeal cancer cells(P<0.05).Importantly MYCT1 significantly inhibited the proliferation,clone formation,and migration of laryngeal carcinoma cells,in a PRSS21 dependent fashion(P<0.05).Conclusion MYCT1 represses the proliferation and migration of laryngeal carcinoma cells via the inhibition of PRSS21 expression.

关 键 词：MYCT1 PRSS21 喉癌 增殖 迁移 

分 类 号：R739.6[医药卫生—肿瘤]