超高效液相色谱-串联质谱法测定茶树新梢中两种香叶醇糖苷含量  

UHPLC-MS/MS Determination of Geraniol Glycosides in Tea Shoots

作　　者：王让剑[1] 苏德森 吴建衍 黄崇耀 陈立松 WANG Rang-jian;SU De-sen;WU Jian-yan;HUANG Chong-yao;CHEN Li-song(Tea Research Institute,Fujian Academy of Agricultural,Fuzhou Fujian 350013,China;Institute of Quality Standards and Testing Technology for Agro-products,Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-products Qualitiy&Safety,Fuzhou,Fujian 350003,China;Shimadzu(China)Co,Ltd,Guangzhou Branch,Guangzhou Guangdong 510010,China)

机构地区：[1]福建省农业科学院茶叶研究所,福建福州350013 [2]福建省农业科学院农业质量标准与检测技术研究所/福建省农产品质量安全重点实验室,福建福州350003 [3]岛津企业管理(中国)有限公司广州分公司,广东广州510010

出　　处：《茶叶学报》2020年第3期114-119,共6页Acta Tea Sinica

基　　金：福建省属公益类科研院所基本科研专项(2018R1012-3);福建省农业科学院“十三五”茶树种质资源青年创新团队(STIT2017-3-12);福建省现代农业(茶叶)产业技术体系(闽农综2019〔144号〕)。

摘　　要：应用超高效液相色谱-串联质谱技术,建立了茶树新梢中香叶醇葡萄糖苷、香叶醇樱草糖苷的检测方法,并测定了6个茶树品种中的两种香叶醇糖苷的含量。通过对前处理条件优化,获得最优条件为:色谱柱采用C18柱(2.0μm,2.1 mm×100 mm),梯度洗脱,流动相A为0.1%(V/V)甲酸水溶液,流动相B为乙腈,流速0.3 mL·min-1,柱温40℃,质谱采用ESI负离子模式,两种化合物峰形好、分离度和灵敏度高。香叶醇葡萄糖苷、香叶醇樱草糖苷的线性范围良好(r2≥0.9958),检出限分别为44.7μg·kg^-1、22.3μg·kg^-1,定量限分别为149.0μg·kg^-1、74.3μg·kg^-1,平均回收率分别为97.1%、98.1%,精密度分别为8.13%、5.07%。参试6个茶树品种新梢不同部位均检出了香叶醇葡萄糖苷与香叶醇樱草糖苷,两种糖苷的含量均随新梢成熟度增加而下降,相同品种新梢中香叶醇樱草糖苷含量高于香叶醇葡萄糖苷含量,适制乌龙茶、高香红茶品种新梢中两种糖苷的含量高于适制绿茶品种。A UHPLC-MS/MS method was developed to determine the contents of geraniol glucoside and geraniol primrose glucoside in tea shoots from various tea cultivars.Pre-treatment and test conditions for the determination were optimized to include the use of a C18 column(2.0μm,2.1mm×100mm)and gradient elution with 0.1%(V/V)formic acid aqueous solution as mobile phase A and acetonitrile as mobile phase B under a flow rate of 0.3mL/min at a column temperature of 40℃.The ESI negative ion mode applied for the mass spectrometry yielded distinct peaks on the two compounds with high resolution and sensitivity.Linearities of the working curves on both glycosides was obtained with r2≥0.9958 at the detection limits of 44.7μg/kg and 22.3μg/kg and the quantitative limits of 149.0μg/kg and 74.3μg/kg.The average recoveries on the two glycosides were 97.1%and 98.1%,and the average precisions 8.13%and 5.07%.Both glucosides were detected in parts of the new shoots of 6 tea varieties with decreasing contents in the maturing shoots.Within a same tea variety,the content of geraniol primrose glucoside was higher than that of geraniol glucoside.The high glycosides content in tea shoots are desirable for the making of oolong or aromatic black teas but not suitable for green tea.

关 键 词：茶树 香叶醇 糖苷 超高效液相色谱-串联质谱 

分 类 号：S571.1[农业科学—茶叶生产加工]