Mitochondria determine the sequential propagation of the calcium macrodomains revealed by the super-resolution calcium lantern imaging  

作　　者：Yulin Zhang Jianyong Wang Shijia Xing Liuju Li Shiqun Zhao Wenzhen Zhu Kuo Liang Yanmei Liu Liangyi Chen 

机构地区：[1]State Key Laboratory of Membrane Biology,Beijing Key Laboratory of Cardiometabolic Molecular Medicine,Institute of Molecular Medicine,Peking University,Beijing 100871,China [2]PKU-IDG/McGovern Institute for Brain Research,Beijing 100871,China [3]Department of General Surgery,Xuanwu Hospital,Capital Medical University,Beijing 100053,China [4]Institute for Brain Research and Rehabilitation(IBRR),Guangdong Key Laboratory of Mental Health and Cognitive Science,South China Normal University,Guangzhou 200062,China

出　　处：《Science China(Life Sciences)》2020年第10期1543-1551,共9页中国科学（生命科学英文版）

基　　金：Supported by the grants from the National Science and Technology Major Project Program(2016YFA0500400);the National Natural Science Foundation of China(81925022,91854112,31327901,31521062,31570839,91750203);and Beijing Natural Science Foundation(L172003,7182063).

摘　　要：Despite the wide application of super-resolution(SR)microscopy in biological studies of cells,the technology is rarely used to monitor functional changes in live cells.By combining fast spinning disc-confocal structured illumination microscopy(SD-SIM)with loading of cytosolic fluorescent Ca2+indicators,we have developed an SR method for visualization of regional Ca2+dynamics and related cellular organelle morphology and dynamics,termed SR calcium lantern imaging.In COS-7 cells stimulated with ATP,we have identified various calcium macrodomains characterized by different types of Ca2+release from endoplasmic reticulum(ER)stores.Finally,we demonstrated various roles of mitochondria in mediating calcium signals from different sources;while mitochondria can globally potentiate the Ca2+entry associated with store release,mitochondria also locally control Ca2+release from the neighboring ER stores and assist in their refilling processes.

关 键 词：super-resolution imaging ER Ca2+release MITOCHONDRIAL sequential response 

分 类 号：Q244[生物学—细胞生物学]