A smart DNA nanodevice for ATP-activatable bioimaging and photodynamic therapy  

作　　者：Bei Liu Rui Ma Jian Zhao Yuliang Zhao Lele Li 

机构地区：[1]School of Science,Minzu University of China,Beijing 100081,China [2]CAS Key Laboratoty for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience,National Center for Nanoscience and Technology,Beijing 100190,China [3]College of Materials Science and Optoelectronic Technology,University of Chinese Academy of Sciences,Beijing 100049,China [4]GBA Research Innovation Institute for Nanotechnology,Guangdong 510700,China

出　　处：《Science China Chemistry》2020年第10期1490-1497,共8页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21822401,21805060,21771044);the Young Thousand Talented Program。

摘　　要：Rational design of activatable photosensitizers for controlled generation of singlet oxygen remains a challenge for precise photodynamic therapy(PDT).Herein,we present an aptamer-based nanodevice for adenosine 5′-triphosphate(ATP)-activatable bioimaging and PDT.The nanodevice is constructed by modifying ATP-responsive duplex DNA units and polyethylene glycol on the surface of a gold nanoparticle(AuNP)through the thiolate-Au chemistry.The DNA units were designed by the hybridization of the ATP aptamer strand with a methylene blue(MB)-modified complementary DNA(cDNA).The close proximity of MB to the surface of AuNP results in the low photodynamic activity of MB(OFF state).Once internalized into cancer cells,the ATP-binding induced conformation switch of aptamer strand leads to the release of the MB-bearing DNA strand from AuNPs,resulting in the activatable generation of singlet oxygen under light irradiation(ON state).We demonstrate that the DNA nanodevice represents a promising platform for ATP-responsive bioimaging and specific PDT in vitro and in vivo.This work highlights a potential way for specific tumor diagnosis and therapy.

关 键 词：DNA nanotechnology ATP-activatable photodynamic therapy BIOIMAGING 

分 类 号：R730[医药卫生—肿瘤] TB383.1[医药卫生—临床医学]