单核细胞增生李斯特菌lmo0732基因克隆、分子特征及遗传进化分析  

Cloning, Molecular Characteristics and Phylogenetic Analysis of the lmo0732 Gene of Listeria monocytogenes

作　　者：李杰[1] 张星星[2] 乔梦凡 孟庆玲[1] 乔军[1] 李妍 王晓婷 李静[1] 才学鹏[3] LI Jie;ZHANG Xingxing;QIAO Mengfan;MENG Qingling;QIAO Jun;LI Yan;WANG Xiaoting;LI Jing;CAI Xuepeng(College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang 832003,China;State Key Laboratory for Sheep Genetic Improvement and Healthy Production,Xinjiang Academy of Agricultural and Reclamation Science,Shihezi,Xinjiang 832000,China;Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu 730046,China)

机构地区：[1]石河子大学动物科技学院,新疆石河子832003 [2]新疆农垦科学院,省部共建绵羊遗传改良与健康养殖国家重点实验室,新疆石河子832000 [3]中国农业科学院兰州兽医研究所,甘肃兰州730046

出　　处：《家畜生态学报》2020年第9期18-23,共6页Journal of Domestic Animal Ecology

基　　金：国家国际科技合作专项(2013DFR31310);国家自然科学基金(31360596,30960274);省部共建绵羊遗传改良与健康养殖国家重点实验室优秀中青年人才培养引导计划专项(SKLSGIHP2017A03)。

摘　　要：为揭示单核细胞增生李斯特菌(LM)lmo0732基因的分子特征及生物学功能,对LM lmo0732基因进行扩增、克隆和测序,利用在线软件对其进行分子特征及遗传进化分析。结果表明,lmo0732基因全长为1 917 bp,编码638个氨基酸;氨基酸序列分析发现,该蛋白为稳定的亲水性蛋白,含有1个跨膜区域,存在信号肽结构;蛋白结构域预测发现该蛋白含有Big 3、MucBp和LRR保守结构域;二、三级结构分析发现,该蛋白为无规则卷曲连接多段延伸链及α-螺旋所形成的三维立体结构。遗传进化分析显示,不同地域分离株可分为两个基因群,表现出一定的遗传多样性。In order to reveal the molecular characteristics and biological function of lmo0732, lmo0732 gene was amplified and cloned, and the sequence was analyzed by bioinformatics software and conducted to construct phylogenetic tree in this study. The results showed that the lmo0732 gene was successfully amplified with the length of 1917 bp and encoded 638 aa. Amino acid sequence analysis found that the Lmo0732 protein was a stable protein with hydrophilicity;it had a signal peptide and contained transmembrane domain. Protein domain prediction showed that the Lmo0732 protein included Big 3, MucBp and LRR domains;analysis on secondery and tertiary structure found that Lmo0732 protein was a three-dimensional structure formed by random coils connecting multiple extended strands and alpha helices. Phylogenetic analysis showed that isolates from different geographic regions could be divided into two gene groups and presented genetic diversity.

关 键 词：单增李斯特菌 lmo0732基因 克隆 序列分析 

分 类 号：S811.5[农业科学—畜牧学]