基于网络药理学探讨枣仁安神颗粒治疗失眠的作用机制  

Anti-insomnia effect of Zaoren Anshen Granules based on network pharmacology

作　　者：杨雪[1,2] 刘传鑫[2] 袁付丽 李梦雨 王文鑫 陈亚红 黄建梅 YANG Xue;LIU Chuanxin;YUAN Fuli;LI Mengyu;WANG Wenxin;CHEN Yahong;HUANG Jianmei(Department of Pharmacy,Changzhi Medical College,Changzhi 046000,China;School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China;State Key Laboratory of Antitoxic Drugs and Toxicology,Beijing Key Laboratory of Neuropsychopharmacology,Institute of Toxicology,Academy of military medicine,Academy of Military Sciences,Beijing 100850,China)

机构地区：[1]长治医学院药学系,山西长治046000 [2]北京中医药大学中药学院,北京100029 [3]军事科学院军事医学研究所毒物药物研究所抗毒药物与毒理学国家重点实验室神经精神药理学北京市重点实验室,北京100850

出　　处：《药物评价研究》2020年第9期1780-1786,共7页Drug Evaluation Research

基　　金：国家自然科学基金资助项目(81173541)。

摘　　要：目的基于网络药理学探讨枣仁安神颗粒治疗失眠的作用机制。方法通过查阅相关文献,挖掘枣仁安神颗粒的入血成分,并采用反向分子对接,预测入血成分的相关靶点,与失眠靶点进行交集映射,获得其治疗失眠的作用靶点。利用蛋白互作筛选核心靶点,并进行分子对接验证。基于共有靶点,采用功能注释生物信息学芯片进行分析,得到枣仁安神颗粒治疗失眠的关键通路。结果方中3味中药酸枣仁、丹参、北五味子共得到29个入血成分和184个潜在靶点,其中19个成分、60个靶点和36条通路与失眠极为密切,主要涉及神经活性配体-受体相互作用、色氨酸代谢、5-羟色胺能突触、GABA能突触等相关分子环节。结论方中3味中药以作用靶点为媒介,连接入血成分及相关通路,从而发挥抗失眠的生物学效应,体现了中药多成分、多靶点,多途径协同起效的作用特点。Objective The mechanism of the treatment of insomnia by Zaoren Anshen Granules was discussed based on the network pharmacology.Methods By consulting the relevant literatures,the blood components of Zaoren anshen granules were excavated.Reverse molecular docking was used to predict the relevant targets of chemical components,Potential targets of Zaoren anshen granules were obtained by mapping with targets of insomnia.Subsequently,protein-protein interactions were used to screen core targets and molecular docking validation was performed.Based on the above-mentioned common targets,functional annotation bioinformatics chips were used to analyze the key pathways for the treatment of insomnia by Zaoren anshen granules,and to explore the possible mechanism of treatmenting insomnia.Results Three traditional Chinese medicines,29 blood components and 184 potential targets were obtained,of which 19 components and 60 targets and 36 pathways were related to insomnia,mainly related to neuroactive ligand-receptor interaction,tryptophan metabolism,serotonin synapses,GABA ergic synapses and other related molecular links.Conclusion The targets were used as a medium to connect blood components and related pathways,thus exerting the biological effect of insomnia,reflecting the multi-components,multi-targets and multi-pathways synergistic action of traditional Chinese medicine.

关 键 词：网络药理学 分子对接 枣仁安神颗粒 失眠 作用靶点 分子机制 

分 类 号：R285.5[医药卫生—中药学]