Vitrification of In vitro-matured Oocytes:Effects of Meiotic Spindle Morphology on Clinical Outcome  

作　　者：Rui-Huan Gu Zhi-Chao Li Jing-Wen Lang Hua Chen Yun Feng Song Guo Jing Fu Xiao-Xi Sun Yi-Juan Sun 

机构地区：[1]Shanghai Ji Ai Genetics and IVF Institute,Obstetrics and Gynecology Hospital,Fudan University,Shanghai 200011,China [2]Center for Reproductive Medicine,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200120,China [3]Reproductive Medical Center,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China [4]Department of Gynaecology and Obstetrics,Qianfoshan Hospital,Shandong University,Jinan 250014,China [5]Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases,Shanghai 200011,China

出　　处：《Reproductive and Developmental Medicine》2020年第1期18-24,共7页生殖与发育医学（英文版）

基　　金：Natural Science Foundation of China(No.81601342,81901558)from Dr.Yi-Juan Sun and Rui-huan Gu;Shanghai Municipal Planning Commission of Science and Research Fund(General Program,No.201740075);Huangpu District,Shanghai Municipal Planning Commission of Science and Research Fund(No.HKW201659).

摘　　要：Objective:The meiotic spindle controls chromosome movement and mediates various functions essential for fertilization and early postfertilization events.This study aimed to examine whether vitrification causes meiotic damage in vitro-matured metaphase II(MII)human oocytes,and whether the meiotic spindle morphology influences the subsequent developmental outcomes.Methods:The spindle characteristics of MII human oocytes in vitro matured were studied before and after vitrification using PolScope imaging and immunofluorescence staining.The developmental competence of oocytes was also examined.Results:A total of 419 human MII oocytes were obtained from 593 intracytoplasmic sperm injection cycles at our hospital.Of these oocytes,54 were used for immunofluorescence staining,whereas the other oocytes were examined by PolScope imaging and classified into three groups according to the meiotic spindle morphology:(A)normal morphology,(B)weak refraction and short meiotic spindle,and(C)no detectable meiotic spindle.The three groups demonstrated statistically significant differences in terms of survival after vitrification.However,differences were not found in terms of oocyte chromosome structure and meiotic spindle morphology on immunofluorescence staining performed before and after vitrification.Oocyte survival,fertilization,and early embryonic development rates were significantly higher in Group A than in Groups B and C with or without vitrification.While vitrification had no effect on these metrics in Group A,Groups B and C demonstrated significantly lower fertilization and cleavage rates after vitrification/warming.Conclusions:Screening for normal meiotic spindle morphology and chromosome configuration before vitrification may increase the yield of healthy viable oocytes for various assisted reproductive technologies.

关 键 词：Immunofluorescence Staining Meiotic Spindle OOCYTES PolScope Imaging VITRIFICATION 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]