不同强度净风、风沙流吹袭对黑果枸杞叶片生理指标的影响  

Effects of net-wind and wind-sand flow on the physiology of Lycium ruthenicum

作　　者：杨永义 马彦军[1] 魏林源[2] 马瑞[1] 侍新萍 张莹花[2] 唐卫东[2] YANG Yongyi;MA Yanjun;WEI Linyuan;MA Rui;SHI Xinping;ZHANG Yinghua;TANG Weidong(Forestry College,Gansu Agricultural University,Lanzhou 730070,Gansu,China;State Key Laboratory Breeding Base of Desertification and Aeolian Sand Disaster Combating,Gansu Desert Control Research Institute,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃农业大学林学院,甘肃兰州730070 [2]甘肃省治沙研究所荒漠化与风沙灾害防治重点实验室,甘肃兰州730070

出　　处：《草业科学》2020年第9期1795-1802,共8页Pratacultural Science

基　　金：国家自然科学基金项目(31760244、31560215);甘肃省青年基金项目(17JR5RA013);甘肃农业大学青年研究生指导教师扶持基金项目(GAU-QNDS-201712);甘肃省GEF/OP12三期项目专题研究项目(GS-GEF/OP12-02);甘肃农业大学科技创新项目“林学一级学科建设开放基金”(GAU-XKJS-2018-112);中国博士后科学基金(2017M613295XB)。

摘　　要：以黑果枸杞(Lycium ruthenicum)一年实生苗为材料,研究0(CK)、6、9、12、15 m·s^–15个风速下净风和风沙流吹袭对黑果枸杞叶片丙二醛(MDA)含量、膜透性、酶活性、渗透调节物质含量的影响,探索黑果枸杞对净风和风沙流胁迫的生理响应机制。结果表明:净风处理下,MDA含量、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、可溶性蛋白含量、可溶性糖含量均逐渐降低,膜透性、过氧化物酶(POD)活性均逐渐增大,脯氨酸含量先上升后下降。风沙流处理下,MDA含量、POD活性、可溶性糖含量均呈先上升后下降的变化趋势,其余指标的变化趋势与净风处理一致。净风和风沙流处理均对黑果枸杞叶片造成一定程度的伤害,黑果枸杞受胁迫时通过POD活性的提高增强保护作用;净风胁迫下,主要通过脯氨酸含量的提高增强渗透调节作用;风沙流胁迫下,主要通过脯氨酸和可溶性糖含量的提高增强渗透调节作用。The present study investigated the effects of net-wind and wind-sand flow on the malondialdehyde content,membrane permeability,protective enzyme activity,and osmoregulation substance content of Lycium ruthenicum leaves with wind speeds of 0(CK),6,9,12,and 15 m·s^–1.The net-wind treatment,malondialdehyde content,superoxide dismutase(SOD)activity,catalase(CAT)activity,soluble protein content,and soluble sugar content decreased,whereas membrane permeability and peroxidase(POD)activity increased,and proline content increased first and then decreased.Under the wind-sand flow treatment,malondialdehyde content,POD activity,and soluble sugar content all increased first and then decreased,whereas the trends of other indicators were identical to those of the net-wind treatment.Both the net-wind and wind-sand flow treatments damaged the leaves,and this stress caused L.ruthenicum to increase POD activity and to enhance osmotic regulation,either by eliciting increases in proline content(net-wind flow stress)or by eliciting increases in both proline and soluble sugar contents(wind-sand flow stress).

关 键 词：渗透调节物质 生理响应 风沙流胁迫 膜脂过氧化作用 

分 类 号：S567.19[农业科学—中草药栽培]