长链非编码RNA-HIT在膀胱癌组织的表达及其对膀胱癌细胞生物学的影响  

Expression of the long non-coding RNA HIT in human bladder cancer tissues and its effect on bladder cancer cell biology

作　　者：万锋 曹辉[3] 杨霞 冯科 曲晓伟 夏彦清 郭海彬 张翠莲 Wan Feng;Cao Hui;Yang Xia;Feng Ke;Qu Xiaowei;Xia Yanqing;Guo Haibin;Zhang Cuilian(Henan Provincial People’s Hospital,Henan Provincial Reproductive Hospital,People’s Hospital of Zhengzhou University,People’s Hospital of Henan University,Zhengzhou 450003,China;Department of Urology,the Central Hospital of Wuhan,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430014,China;Department of Orthopaedics,People’s Hospital of Wuhan University,Wuhan 430200,China;Department of Gastroenterology,Wuhan Asia General Hospital,Wuhan 430056,China)

机构地区：[1]河南省人民医院河南省生殖医院郑州大学人民医院河南大学人民医院,450003 [2]华中科技大学同济医学院附属武汉中心医院泌尿外科,430014 [3]武汉大学人民医院东院骨科,430200 [4]武汉亚心总医院消化内科,430056

出　　处：《中华实验外科杂志》2020年第8期1401-1404,共4页Chinese Journal of Experimental Surgery

基　　金：湖北省自然科学基金(2016CFB596);武汉市卫计委资助项目(WX16E30)。

摘　　要：目的观察人膀胱移行细胞癌(BTCC)患者肿瘤组织及膀胱癌细胞株中长链非编码RNA(lncRNA)HIT的表达,探讨lncRNA-HIT对膀胱癌细胞的生物学影响。方法应用实时定量反转录聚合酶链反应(RT-qPCR)检测2016年9月至2019年3月华中科技大学同济医学院附属武汉中心医院54例BTCC患者手术切除的癌组织及其配对毗邻正常黏膜组织中lncRNA-HIT的表达水平;统计分析其表达水平与患者临床病理特征之间的相关性。采用短发卡RNA(shRNA)干扰lncRNA-HIT,观察其对膀胱癌细胞的凋亡、增殖及侵袭等生物学的影响。两组均数间比较用t检验,多组间均数比较采用单因素方差分析。结果BTCC患者癌组织中lncRNA-HIT的表达量较配对癌旁正常组织上调(t=5.763,P<0.05);lncRNA-HIT在膀胱癌细胞株EJ、UMUC3和T24细胞的表达较正常膀胱细胞系SV-HUC-1细胞明显上调(t=15.030、13.020、14.050,P<0.05)。lncRNA-HIT异常增高与患者肿瘤分级(χ^2=6.862,P<0.01)、分期(χ^2=14.814,P<0.01)、淋巴结转移(χ^2=9.429,P<0.01)、远处转移(χ^2=12.523,P<0.01)等密切相关,但与性别(χ^2=0.441,P>0.05)和年龄(χ^2=0.106,P>0.05)无明显相关,体外靶向干扰实验显示下调lncRNA-HIT可以显著促进膀胱癌细胞的凋亡,抑制膀胱癌细胞增殖和其侵袭能力。结论lncRNA-HIT在BTCC患者肿瘤组织中显著增高。Objective To investigate the expression of long non-coding RNA(lncRNA)HIT in tumor tissues of bladder transitional cell cancer(BTCC)and its role in the biological behavior of cancerous cells.Methods The expression level of lncRNA-HIT in tumor tissues and adjacent non-tumor tissues of BTCC patients from surgically resected was detected by real-time quantitative polymerase chain reaction(Real-time PCR),and its correlation with clinicopathological characteristics was analyzed.The lncRNA-HIT was knocked down in UMUC3 and T24 cell lines to investigate its biological effects on apoptosis,proliferation and invasion of bladder cancer cells.Results The expression of lncRNA-HIT in tumor tissues of BTCC patients was significantly increased as compared with that in adjacent non-tumor tissues(t=5.763,P<0.05).The expression of lncRNA-HIT in bladder cancer cell lines EJ,UMUC3 and T24 was significantly higher than that in normal bladder cell lines SV-HUC-1(t=15.030,13.020,14.050,P<0.05).We also found that the high expression of lncRNA-HIT was significantly associated with tumor differentiation(G1 vs.G2+G3,χ^2=6.862,P<0.01),stage(Ta,T1 vs.T2-T4,χ^2=14.814,P<0.01),presence of lymph node metastasis(χ^2=9.429,P<0.01)and presence of distant metastasis(χ^2=12.523,P<0.01).However,no significant differences were observed in lncRNA-HIT expression among patients with different gender(male vs.female,χ^2=0.441,P>0.05)or age(≥55 years vs.<55 years,χ^2=0.106,P>0.05).Knockdown of lncRNA-HIT could promote the apoptosis capability,and inhibit the capability of proliferation and invasion of bladder cancer cells.Conclusion lncRNA-HIT is significantly highly expressed in tumor tissues of BTCC patients.

关 键 词：膀胱移行细胞癌 长链非编码RNA-HIT 凋亡 增殖 侵袭 

分 类 号：R737.14[医药卫生—肿瘤]