基于生物信息学分析筛选三阴性乳腺癌相关的预后标志物  

Identification of hub genes as prognostic biomarkers for triple-negative breast cancer using integrating bioinformatics analysis

作　　者：杨丽洁 杨铁成 石丽稳 王华桥 王丹雯 李炫飞 谢伟[1] Yang Lijie;Yang Tiecheng;Shi Liwen;Wang Huaqiao;Wang Danwen;Li Xuanfei;Xie Wei(Department of Gastrointestinal Surgery,Zhongnan Hospital of Wuhan University,Clinical Medical Research Center of Peritoneal Cancer of Wuhan,Clinical Cancer Study Center of Hubei Province,Key Laboratory of Tumor Biological Behavior of Hubei Province,Wuhan 430071,China)

机构地区：[1]武汉大学中南医院胃肠外科,武汉市腹膜癌临床医学研究中心,湖北省肿瘤医学临床研究中心,肿瘤生物学行为湖北省重点实验室,430071

出　　处：《中华实验外科杂志》2020年第8期1505-1508,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(81770283);武汉市腹膜癌临床医学研究中心资助项目(2015060911020462);武汉大学中南医院创新培育基金(znpy2018004)。

摘　　要：目的分析三阴性乳腺癌(TNBC)与非三阴性乳腺癌(non-TNBC)的基因表达谱,寻找可能参与TNBC发生发展的差异表达基因(DEGs)。方法从GEO数据库下载4个mRNA芯片数据集进行分析,获得基因表达谱。通过STRING和Cytoscape构建了DEGs的蛋白-蛋白相互作用(PPI)网络,并筛选出核心基因。京东基因组百科全书(KEGG)和基因本体论(GO)用于功能富集分析。使用UCSC、Oncomine和Kaplan-Meier在线网站来分析核心基因的表达和生存水平。结果在TNBC和non-TNBC中共识别出287个DEGs和10个核心基因。Kaplan-Meier生存曲线显示,TNBC患者表皮生长因子受体(EGFR)高表达或ESR1、胰岛素样生长因子(IGF)-1低表达与更差预后相关。Non-TNBC患者的预后特征与TNBC患者差异有统计学意义(P<0.05)。结论ESR1、EGFR和IGF-1可能导致TNBC的不良预后与复发。Objective This study aims to search for differentially expressed genes(DEGs),which may be involved in the pathogenesis of triple-negative breast cancer(TNBC)through analysis of gene expression profiles of TNBC compared with non-TNBC.Methods Four mRNA microarray datasets from gene expression omnibus(GEO)were downloaded and analyzed to obtain DEGs.The protein-protein interaction(PPI)network of DEGs was constructed,and hub genes were filtered by STRING and Cytoscape.Bioinformatics analysis was used for functional enrichment analyses,including gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG).UCSC,ClueGO,Oncomine,and Kaplan-Meier plotter online websites are used to analyze expression,and survival levels of hub genes.Results A total of 287 DEGs and 10 hub genes were recognized between TNBC and non-TNBC.Kaplan-Meier survival curves indicated that higher expression of epidermal growth factor receptor(EGFR)or lower expression of ESR1 and insulin-like growth factor(IGF)-1 were correlated with poorer prognosis in TNBC patients.Patients with non-TNBC exhibited prognosis characteristics significantly differing from those with TNBC.Conclusion ESR1,EGFR and IGF-1 might have been involved in poor prognosis and recurrence of TNBC.

关 键 词：三阴性乳腺癌 差异表达基因 生物标志物 预后 

分 类 号：R737.9[医药卫生—肿瘤] Q811.4[医药卫生—临床医学]