血清中长链非编码RNA-ATB在乳腺癌诊断中的意义  

Significance of detection of long-chain non-coding RNA-ATB in serum in the diagnosis of breast cancer

作　　者：洪宏 喻海忠 袁建芬 赵枰[2] HONG Hong;YU Haizhong;YUAN Jianfen;ZHAO Ping(Department of Laboratory Medicine,Nantong Hospital of Traditional Chinese Medicine,Jiangsu 226001,China;Department of Laboratory Medicine,Nantong First People′s Hospital,Jiangsu 226001,China)

机构地区：[1]江苏省南通市中医院检验科,226001 [2]江苏省南通市第一人民医院检验科,226001

出　　处：《重庆医学》2020年第19期3255-3258,3262,共5页Chongqing medicine

基　　金：南通市科技发展计划项目(MSZ18133)。

摘　　要：目的探讨长链非编码RNA(LncRNA)-ATB在乳腺癌患者血清中的表达水平及其对乳腺癌的诊断价值。方法采用定量逆转录PCR(qRT-PCR)检测37例乳腺癌患者、30例乳腺良性疾病患者和26例体检健康者血清中LncRNA-ATB的表达水平,分析血清中LncRNA-ATB的表达水平与乳腺癌临床病理参数的相关性;应用化学发光法检测血清CA153水平,并用受试者工作特征曲线分析单一和联合检测对乳腺癌的诊断价值。结果乳腺癌患者血清中LncRNA-ATB相对表达水平均显著高于乳腺良性疾病患者和健康对照者(P<0.01);乳腺癌患者血清中LncRNA-ATB的表达水平与雌激素受体(ER)、c-erbB-2、Her2、TNM分期,以及淋巴结转移相关(P<0.05);LncRNA-ATB单独检测的受试者工作特征曲线(ROC曲线)下面积(AUC)为0.824(95%CI:0.735~0.914),灵敏度为89.2%,特异度为67.9%;LncRNA-ATB和CA153联合检测的ROC曲线下的AUC为0.876(95%CI:0.800~0.953),灵敏度为83.8%,特异度为80.4%。结论乳腺癌患者血清中LncRNA-ATB水平呈高表达,可能是乳腺癌诊断的一种潜在的生物标志物。Objective To explore the expression level of long non-coding RNA(LncRNA)-ATB in the serum of breast cancer patients and its potential diagnostic value for breast cancer.Methods Quantitative reverse transcription PCR(qRT-PCR)was used to detect the expression level of LncRNA-ATB in the serum of 37 breast cancer patients,30 patients with benign breast diseases,and 26 healthy people on physical examination,and analyze the serum LncRNA-ATB expression and breast cancer For the correlation of clinical pathological parameters,chemiluminescence method was used to detect serum CA153 level,and receiver operating characteristic curve was used to analyze the diagnostic value of single and combined detection for breast cancer.Results The relative expression of LncRNA-ATB in the serum of breast cancer patients was significantly higher than that in patients with benign breast diseases and healthy controls(P<0.01).Moreover,The expression level of LncRNA-ATB in the serum of breast cancer patients is related to estrogen receptor(ER),c-erbB-2,Her2,TNM staging and lymph node metastasis(P<0.05).The area under the receiver operating characteristic curve(ROC curve)(AUC)for the detection of LncRNA-ATB alone was 0.824(95%CI:0.735-0.914),the sensitivity was 89.2%,and the specificity was 67.9%.The AUC under the ROC curve combined with LncRNA-ATB and CA153 detection is 0.876(95%CI:0.800-0.953),the sensitivity is 83.8%,and the specificity is 80.4%.The diagnostic value of combined testing is higher than that of single testing.Conclusion The high expression of serum LncRNA-ATB in breast cancer patients may provide a potential biological marker for the diagnosis of breast cancer.

关 键 词：长链非编码RNA ATB 乳腺癌 生物学标志物 

分 类 号：R737.9[医药卫生—肿瘤]