3,5-二硝基水杨酸比色法测定大米中水溶性糖含量  

Determination of Water-Soluble Sugar in Rice by 3,5-Dinitrosalicylic Acid Colorimetric Method

作　　者：王莉丽 梅文泉[1] 陈兴连[1] 方海仙[1] 刘兴勇[1] 杨东顺[1] 王卫清 Wang Lili;Mei Wenquan;Chen Xinglian;Fang Haixian;Liu Xinyong;Yang Dongshun;Wang Weiqing(Quality Standard and Testing Technology Institute,Yunnan Academy of Agricultural Science,Supervision&Testing Center for Farm Products Quality,Ministry of Agriculture and rural areas,Kunming Section,Kunming 650205)

机构地区：[1]云南省农业科学院质量标准与检测技术研究所,农业农村部农产品质量监督检验测试中心,昆明650223

出　　处：《中国粮油学报》2020年第9期168-173,共6页Journal of the Chinese Cereals and Oils Association

基　　金：云南省创新团队计划(2015HC025)。

摘　　要：建立了大米中水溶性糖的测定方法。大米中的水溶性糖用热水提取,乙酸锌、亚铁氰化钾溶液沉淀,经盐酸水解后用3,5-二硝基水杨酸显色,分光光度计比色测定。结果表明,在3,5二硝基水杨试剂用量1.0 mL,沸水浴加热6 min,540 nm波长条件下进行测定,标准曲线线性关系良好,回归方程Y=0.9682 X+0.0144,相关系数R 2=0.9996,在0.1%、0.5%和2.0%三个标准添加浓度下,水溶性糖的平均加标回收率为91.9%~98.3%,相对标准偏差为2.68%~2.79%,方法检出限为0.003%。该方法准确度和灵敏度高,重现性好,操作简单、快速,有应用价值。10份不同品种大米水溶性糖含量测定结果为0.12%~1.95%。A method for determination of water-soluble sugar in rice was established.The water-soluble sugar in rice was extracted by hot water,and purified with zinc acetate dehydrate and potassium ferrocyanide.The water-soluble sugar in rice was coloration with 3,5-dinitrosalicylic acid,and analysis with spectrophotometer colorimeter.The result indicated that,under the conditions of 1 mL 3,5-dinitrosalicylic acid usage,6 minutes boiling water heating,540 nm wavelength determination,the standard curves had good linearity,linear regression equation was Y=0.9682X+0.0144,correlation coefficient R 2=0.9996.Under adding concentration of 0.1%,0.5%,2.0%,the average recoveries were 91.9%~98.3%,the relative standard deviation was 2.68%~2.79%.The detection limit of this method was 0.003%.The established method has the advantages of simply,rapidity,high accuracy and sensitivity,and was suitable for the determination of water-soluble sugar in rice.The determination results of water-soluble sugar in 10 rice varieties were 0.12%~1.95%.

关 键 词：大米 水溶性糖 3 5-二硝基水杨酸 比色法 测定 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程]