食管鳞状细胞癌差异性表达circRNA的初步筛选和分析  

Preliminary screening and analysis of differentially expressed circRNAs in esophageal squamous cell carcinoma

作　　者：张德海 付正 王健 王国君 黄军贤 陈熹[2] ZHANG Dehai;FU Zheng;WANG Jian;WANG Guojun;HUANG Junxian;CHEN Xi(Department of Thoracic Surgery,Jintan Hospital Affiliated to Jiangsu University,Jintan 213200,Jiangsu,China;College of Life Sciences,Nanjing University,Nanjing 210023,China)

机构地区：[1]江苏大学附属金坛医院胸外科,金坛213200 [2]南京大学生命科学学院,南京210023

出　　处：《中国肿瘤生物治疗杂志》2020年第9期1012-1017,共6页Chinese Journal of Cancer Biotherapy

基　　金：常州市金坛区科学技术局社会发展项目(No.KJ201727)。

摘　　要：目的:构建食管鳞状细胞癌的circRNA表达谱,分析差异性表达的circRNA。方法:标本取自2018年6月至2019年2月在江苏大学附属金坛医院胸外科住院的3例食管鳞状细胞癌手术患者,利用高通量测序技术构建circRNA表达谱,检测3对食管鳞状细胞癌组织和癌旁组织中差异表达的circRNA,并运用GO和KEGG技术分析这些circRNA涉及的生物学作用和相关的信号通路。结果:通过构建的表达谱分析比较食管鳞状细胞癌组织与癌旁组织circRNA的表达水平,共发现了905个差异表达的circRNA,其中404个表达上调、501个表达下调,hsa_circ_0004390是上调倍数最大的circRNA(FC=7.9712),novel_circ_0012687是下调倍数最大的circRNA(FC=5.8796)。GO和KEGG分析表明,这些circRNA可能涉及癌细胞的细胞周期、细胞组分、蛋白质结合作用等生物学过程和Hippo、cGMP-PKG等信号通路。结论:食管鳞状细胞癌组织circRNA表达谱分析得到的高度显著差异表达的circRNA似可以作为食管鳞状细胞癌潜在的生物标志物。Objective:To construct a circRNA profile of esophageal squamous cell carcinoma(ESCC)and analyze differentially expressed circRNAs.Methods:Samples were taken from 3 patients with esophageal squamous cell carcinoma who were hospitalized in the Department of Thoracic Surgery,Jintan Hospital,Jiangsu University from June 2018 to February 2019.The circRNA expression profile was constructed by high-throughput sequencing technique,and the circRNA differentially expressed in 3 pairs of esophageal squamous cell carcinoma tissues and adjacent tissues was detected.The biological functions and related signal pathways of these circRNA were analyzed by GO and KEGG techniques.Results:By comparing the expression levels of circRNA between esophageal squamous cell carcinoma and adjacent tissues,905 differentially expressed circRNA were found,of which 404 were up-regulated and 501 were down-regulated.hsa_circ_0004390 was the CIRC RNA with the highest up-regulation factor(FC=7.9712),and novel_circ_0012687 was the one with the highest down-regulation factor.GO and KEGG analysis showed that these circRNA may be involved in biological processes such as cell cycle,cell components and protein binding of cancer cells,and signal pathways such as Hippo and cGMP-PKG.Conclusion:The expression profile analysis of circRNA in esophageal squamous cell carcinoma showed that the signifi-cantly differentially expressed circRNA could be used as a potential biomarker of esophageal squamous cell carcinoma.

关 键 词：食管鳞状细胞癌 环状RNA 差异表达 生物学作用 高通量测序 

分 类 号：R735.1[医药卫生—肿瘤] R730.5[医药卫生—临床医学]