IL-15基因转染人CIK细胞过继转移治疗胃癌小鼠  

Adoptive transfer of human CIK cells with IL-15 gene transfection in the treatment of gastric cancer in mice

作　　者：毛莉艳 张毅[1] 龚宇蓉 陈莺[1] MAO Li-yan;ZHANG Yi;GONG Yu-rong(Department of Digestive Endoscopy Diagnosis and Treatment,Medical College,Shanghai Jiaotong University,Shanghai 200092,China;Department of General Surgery,Xinhua Hospital,Medical College,Shanghai Jiaotong University,Shanghai 200092,China)

机构地区：[1]上海交通大学医学院附属新华医院消化内镜诊治部,上海200092 [2]上海交通大学医学院附属新华医院普外科,上海200092

出　　处：《临床和实验医学杂志》2020年第20期2129-2132,共4页Journal of Clinical and Experimental Medicine

基　　金：上海市科委科研计划项目(编号:16441900302)。

摘　　要：目的分析白细胞介素-15(IL-15)基因转染人CIK细胞过继转移治疗胃癌小鼠的作用。方法制备CIK细胞、IL-15基因转染CIK细胞。选取15只T细胞免疫缺陷小鼠,制备人源胃癌细胞荷瘤小鼠模型。将小鼠分为3组:IL-15转染CIK组(瘤体局部注射IL-15基因转染CIK细胞)、CIK组(瘤体局部注射CIK细胞)、对照组(瘤体局部单纯注射0.9%氯化钠溶液)。连续治疗2周,停止治疗后,观察2周。观察比较3组的小鼠治疗后4周的肿瘤体积、肿瘤形态、抑瘤率。结果①治疗后1周,IL-15-CIK组、CIK组的肿瘤体积明显小于对照组,差异有统计学意义(P<0.05),IL-15-CIK组与CIK组比较,差异无统计学意义(P>0.05)。治疗后第2周,IL-15-CIK组的肿瘤体积开始减少,CIK组、对照组的肿瘤体积继续增加。停止治疗后观察第1周(治疗后第3周),3组的肿瘤体积均有增加,但IL-15-CIK组最少,其次为CIK组,组间比较差异均具有统计学意义(P<0.05)。停止治疗后观察第2周(治疗后第4周),3组肿瘤体积均有减少,IL-15-CIK组肿瘤体积最少,其次为CIK组,组间比较差异均具有统计学意义(P<0.05)。②各组小鼠治疗后第4周,对照组的小鼠腋下存在较大瘤块,个别瘤块出现溃烂。相比对照组,CIK组小鼠存在较小瘤块,有肉眼无法观察发现的肿瘤,处死小鼠后,找到很小的瘤组织。IL-15-CIK组小鼠,治疗后开始,2只小鼠出现肿瘤溃烂。第2周溃烂结痂,第4周溃烂愈合,处死小鼠后,未见瘤组织。③对比对照组,IL-15-CIK组抑瘤率为87.03%,CIK组抑瘤率为82.75%;对比CIK组,IL-15-CIK组抑瘤率为26.89%。IL-15-CIK组的抑瘤能力更高。结论IL-15基因转染人CIK细胞,对胃癌小鼠进行过继转移治疗,能够有效减少肿瘤体积,提高抑瘤率,增强杀瘤作用及延长免疫反应持续时间,提升治疗效果。Objective To analyze the effect of human CIK cell adoptive transfer transfected with IL-15 gene in mice with gastric cancer.Methods CIK cells and IL-15 transfection into CIK cells were prepared.Fifteen T-cell immunodeficient mice were selected to prepare human gastric cancer cell tumor-bearing mouse model.Mice were divided into three groups.One group was the CIK group transfected with IL-15,and CIK cells were transfected with local injection of IL-15 into the tumor.One group was CIK group,and CIK cells were locally injected into the tumor.The first group was the normal saline group,and the tumor body was only injected with the normal saline.The mice were treated continuously for 2 weeks,after stopping the treatment,they were kept under observation for 2 weeks.The tumor volume,tumor morphology and tumor inhibition rate of the three groups were observed and compared.Results①After 1 week of treatment,tumor volume in the IL-15-CIK group and the CIK group was significantly smaller than that in the normal saline group(P<0.05),there was no significant difference between the IL-15-CIK group and the CIK group(P>0.05).After two weeks of treatment,tumor volume began to decrease in the IL-15-CIK group and continued to increase in the CIK group and the saline group(P<0.05).One week(three weeks)after discontinuation of treatment,tumor volume increased in all three groups,but the least in the IL-15-CIK group,followed by the CIK group,with statistically significant differences between the groups(P<0.05).In the second week(4 weeks)after discontinuation of treatment,tumor volume decreased in all three groups,but at least in the IL-15-CIK group,followed by the CIK group,with statistically significant differences(P<0.05).②Four weeks after treatment,large tumor masses were found in the armpit of nude mice in the normal saline group,and some tumor masses were ulcerated.Nude mice in the CIK group had tumor masses,which were smaller than those in the normal saline group.There were tumors that could not be seen by the naked eye.When

关 键 词：小鼠 胃肿瘤 IL-15基因 转染 CIK细胞 过继转移 

分 类 号：R735.2[医药卫生—肿瘤]