活酵母对猪大肠体外产甲烷的抑制作用  

The inhibitory effect of live yeast on methane production in pig large intestine

作　　者：孙秀青[1] 赵红丽 SUN Xiuqing;ZHAO Hongli(Hebi Polytechnic,Hebi,Henan Province 458000,China)

机构地区：[1]鹤壁职业技术学院,河南鹤壁458000

出　　处：《中国饲料》2020年第18期21-24,共4页China Feed

基　　金：2016年度教育教学改革研究项目(2018CG007)。

摘　　要：本研究选择了两珠活酵母用于评估其对猪大肠体外发酵甲烷生成的抑制作用。活酵母的浓度为1.8×1010个/g,两种酵母分别以0.3 mg/mL和0.6 mg/mL的浓度添加,收集2头商品猪的大肠内容物,与缓冲液按照1∶3混合,使用500 mg底物在39℃厌氧培养24 h。结果发现:添加酵母后,总气产量和甲烷产量显著下降(P<0.05),其中面包酵母组甲烷产量占比显著低于对照组(P<0.05)。补充0.6 mg/mL酿酒酵母和0.3 mg/mL面包酵母组丙酸、丁酸、戊酸及挥发性脂肪酸总量浓度显著高于对照组(P<0.05)。0.6 mg/mL面包酵母组以及0.3和0.6 mg/mL酿酒酵母组丙酸、丁酸、戊酸比例显著升高(P<0.05),乙酸比例显著降低(P<0.05)。补充两种酵母显著降低了氢的回收率(P<0.05)。甲烷产量和乙酸占比及乙酸与丙酸比值呈显著正相关(P<0.05)。结论:本研究中使用的2种活酵母是潜在的肠内甲烷生成抑制剂。与对照组相比,酿酒酵母水平越高,面包酵母水平越低,干物质体外降解率、挥发性脂肪酸总量、丙酸产量越高少。In this study,two live yeasts were selected to evaluate their inhibitory effect on methane production from pig large intestine fermentation in vitro.The concentration of live yeast was 1.8×1010/g.The two yeasts were added at concentrations of 0.3 mg/mL and 0.6 mg/mL,respectively.The large intestine contents of 2 commercial pigs were collected and mixed with buffer at 1:3.Results:after adding yeast,the total gas production and methane production decreased significantly(P<0.05),and the proportion of methane production in the baker’s yeast group was significantly lower than that in the control group(P<0.05).The total concentrations of propionic acid,butyric acid,pentanoic acid and volatile fatty acids in the 0.6mg/mL saccharomyces cerevisiae and 0.3mg/mL baker’s yeast group were significantly higher than those in the control group(P<0.05).The proportions of propionic acid,butyric acid and valeric acid in the 0.6mg/mL baker’s yeast group and 0.3 and 0.6mg/mL saccharomyces cerevisiae group were significantly increased(P<0.05),while the proportions of acetic acid were significantly decreased(P<0.05).The addition of two yeasts significantly reduced the hydrogen recovery(P<0.05).There was a significant positive correlation between methane yield,acetic acid ratio and acetic acid and propionic acid ratio(P<0.05).Conclusion:the two live yeasts used in this study are potential intestinal methanogenesis inhibitors.Compared with the control group,the higher the level of saccharomyces cerevisiae,the lower the level of baker’s yeast,the higher the in vitro degradation rate of dry matter,the total volatile fatty acids,and the lower the propionic acid yield.

关 键 词：活酵母 猪 大肠 甲烷 体外发酵 

分 类 号：S828[农业科学—畜牧学]