颈动脉粥样硬化斑块的非标记定量蛋白质组学研究  

Proteomics analysis of distinct proteins in carotid atherosclerotic plaques

作　　者：邹璇[1] 王辰[2] 刘一静 施铭岗 安中平 黄慧玲 周官恩 ZOU Xuan;WANG Chen;LIU Yijing(Department of Neurology,Huanhu Hospital Affiliated to Nankai University,Tianjin 300350,China)

机构地区：[1]南开大学附属环湖医院神经内科,天津300350 [2]南开大学附属环湖医院,天津市神经外科研究所,天津300350 [3]天津医科大学研究生院,天津300070 [4]南开大学附属环湖医院神经外科,天津300350

出　　处：《中风与神经疾病杂志》2020年第9期778-781,共4页Journal of Apoplexy and Nervous Diseases

基　　金：天津市卫生计生委科技基金(No.15KG109)。

摘　　要：目的建立颈动脉粥样硬化不稳定斑块和稳定斑块的表达图谱,进行差异蛋白质组学分析,鉴定出差异表达蛋白,寻找对颈动脉粥样硬化不稳定斑块诊断有特异性和敏感性的生物学标志物。方法依据颈动脉B超检查结果初步分为颈动脉不稳定斑块组、颈动脉稳定斑块组。募集经颈部血管超声检查发现颈动脉狭窄,且做颈动脉内膜剥脱术的缺血性卒中患者20例,分为不稳定斑块组10例和稳定斑块组10例。利用SDS-PAGE酶解后反相色谱Orbitrap Fusion进行蛋白质分析,并质谱数据处理,检索软件,产生的质谱原始文件采用MaxQuant软件处理。结果运用蛋白质组学技术鉴定出差异表达蛋白1240个,在不稳定斑块中,表达下调的蛋白有432个,表达上调的有808个。COG分析显示主要与信号转导、脂质转运和代谢、细胞翻译后修饰、细胞骨架、蛋白酶解等方面相关。结论颈动脉粥样硬化不稳定斑块和稳定斑块的表达图谱是有差别的,进行差异蛋白质组学分析,鉴定出差异表达蛋白,为进一步筛选特异性功能相关蛋白和干预靶位奠定了基础。Objective We used proteomics technologies in an attempt to differentiate and identify histological proteins that were associated with carotid atherosclerotic plaques and further understanding of the pathogenesis.Methods Carotid atherosclerotic plaques were obtained from the patients who undergo carotid endarterectomy in the Tianjin Huanhu Hospital.Plaques divided into two groups:stable group and unstable group.We comparatively analyzed the proteome of 10 unstable plaques and 10 stable plaques.The differentially expressed proteins were visualized by two dimensional electrophoresis(2DE)and were analyzed by a coupled label-free and mass spectrometry approach.The protein identification program was used to search the Uniprot.Results A total of 1240 different proteins were identified by label-free and mass spectrometry.A total of 808 proteins were upregulated,and 432 proteins were downregulated in unstable plaques group compared to stable plaques group.They are referring to signal transduction,lipid transport and metabolism,posttranslational modification,cytoskeleton and Proteolysis.Conclusion Proteomics analysis can be used to investigated differentially expressed proteins.However,Additional confirmatory studies are required to elucidated their role in the atherosclerotic process.

关 键 词：动脉粥样硬化 斑块 蛋白质组学 

分 类 号：R743.1[医药卫生—神经病学与精神病学]