An immunoassay based on lab-on-a-chip for simultaneous and sensitive detection of clenbuterol and ractopamine  

作　　者：Qi Wang Jinqi Deng Yiping Chen Yunjing Luo Xingyu Jiang 

机构地区：[1]College of Life Science and Bioengineering,Beijing University of Technology,Beijing 100124,China [2]Beijing Engineering Research Center for BioNanotechnology and CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,CAS Center for Excellence in Nanoscience,National Center for NanoScience and Technology,Beijing 100190,China [3]Department of Biomedical Engineering,Southern University of Science and Technology.Shenzhen 518055,China [4]University of Chinese Academy of Sciences,Beijing 100049,China [5]College of Food Science and Technology.Huazhong Agricultural University,Wuhan 430070,China

出　　处：《Chinese Chemical Letters》2020年第7期1835-1838,共4页中国化学快报（英文版）

基　　金：the National Key R&D Program of China(Nos.2018YFA0902600,2017YFA0205901);the National Natural Science Foundation of China(Nos.21535001,81730051,21761142006);the Chinese Academy of Sciences(Nos.QYZDJ-SSW-SLH039,121D11KYSB20170026,XDA16020902)for financial support。

摘　　要：Both clenbuterol(CLB)and ractopamine(RAC)areβ-adrenergic agonists.After long-term excessive intake,there will be adverse reactions such as headache,chest tightness,limb numbness,and serious lifethreatening.Simultaneous detection of CLB and RAC in related samples is of great importance for human health.In this work,we outline a microfluidics-based indirect competitive immunoassay(MICI)system that can sensitively detect residual CLB and RAC in pork,swine blood and swine urine.The rapid detection of multiple samples can be achieved in one chip,which greatly improves the detection efficiency.This method has good stability and reproducibility and the microfluidic chips are easy to manufacture.The linear ranges for CLB and RAC detection by MICI are 0.1-2.5 ng/mL and 0.1-5 ng/mL,and the limits of detection(LODs)are 0.094 ng/mL and 0.091 ng/mL,respectively.This straightforward and portable immunoassay system provides a good platform for rapid detection of harmful substances in food samples.

关 键 词：Microfluidic chips Simultaneous detection Indirect competitive immunoassay CLENBUTEROL RACTOPAMINE 

分 类 号：TS207.3[轻工技术与工程—食品科学]