桑叶生物碱对D-半乳糖诱导氧化损伤模型小鼠肾脏的改善作用  

Mulberry Leaf Alkaloids Improve D-Galactose-Induced Oxidative Damage in the Mouse Kidney

作　　者：杨忠敏 沈以红[2] 王祖文 黄先智[2] 丁晓雯 YANG Zhongmin;SHEN Yihong;WANG Zuwen;HUANG Xianzhi;DING Xiaowen(Chongqing Key Laboratory of Agricultural Products Processing,National Demonstration Center for Experimental Food Science and Technology Education,College of Food Science,Southwest University,Chongqing 400716,China;Science and Technology Department,Southwest University,Chongqing 400716,China)

机构地区：[1]西南大学食品科学学院,重庆市农产品加工重点实验室,食品科学与工程国家级实验教学示范中心,重庆400716 [2]西南大学科技处,重庆400716

出　　处：《食品科学》2020年第19期198-203,共6页Food Science

基　　金：现代农业产业技术体系建设专项(CARS-18)。

摘　　要：目的:探讨桑叶生物碱对肾脏氧化损伤是否具有改善作用,旨在为桑叶生物碱的开发利用提供理论依据。方法:采用D-半乳糖诱导建立小鼠氧化损伤模型,然后灌胃不同剂量的桑叶生物碱,于第4周末测定小鼠肾脏组织中蛋白羰基(protein carbonyl,PCO)、晚期蛋白氧化产物(advanced oxidation protein products,AOPP)、8-异前列腺素F(2α)(8-iso-prostaglandin F(2α),8-iso-PGF(2α))、8-羟基鸟嘌呤(8-hydroxy-2’-desoxyguanosine,8-OH-dG)、5-羟基胞嘧啶(5-hydroxy-2’-deoxycotosine,5-OH-dC)的水平及超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、还原型烟酰胺腺嘌呤二核苷酸磷酸(reduced nicotinamide adenine dinucleotide phosphate,NADPH)氧化酶(NADPH oxidase,NOX)、蛋白激酶C(protein kinase C,PKC)的酶活力。结果:与模型对照组相比,桑叶生物碱高剂量组(200 mg/kg mb)能使小鼠肾脏组织中蛋白氧化产物PCO、AOPP分别降低37.27%、42.71%(P<0.01);脂质氧化产物8-iso-PGF(2α)降低51.07%(P<0.01);DNA氧化产物8-OH-dG、5-OH-dC分别降低31.66%、18.91%(P<0.01);同时,桑叶生物碱高剂量组也使肾脏组织中抗氧化酶SOD、GSH-Px活力分别升高83.74%、59.31%(P<0.01);NOX、PKC活力分别降低41.21%、40.65%(P<0.01)。结论:桑叶生物碱对D-半乳糖诱导的小鼠肾脏氧化损伤具有较好改善作用,其机制可能与升高SOD、GSH-Px活力和抑制NOX、PKC活力,进而减少机体活性氧自由基过量产生有关。Objective:To explore whether mulberry leaf alkaloids can improve oxidative damage in the kidney of mice for the purpose of providing a theoretical basis for the development and utilization of mulberry leaf alkaloids.Methods:A mouse model of renal oxidative damage was induced by D-galactose(D-Gal)and the model mice were administered with different doses of mulberry leaf alkaloids for 4 consecutive weeks.At the end of the 4th week,the levels of protein carbonyl(PCO),advanced oxidation protein products(AOPP),8-iso-prostaglandin F2α(8-iso-PGF2α),8-hydroxy-2’-desoxyguanosine(8-OH-dG)and 5-hydroxy-2’-deoxycotosine(5-OH-dC),and the activity of total superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),reduced nicotinamide adenine dinucleotide phosphate oxidase(NOX),and protein kinase C(PKC)in the kidney was measured.Results:Compared with the model control group,high-dose mulberry leaf alkaloids(200 mg/kg mb)could reduce the protein oxidation products PCO and AOPP in the kidney tissues of mice by 37.27%and 42.71%,respectively(P<0.01),the lipid oxidation product 8-iso-PGF2αby 51.07%(P<0.01),and the DNA oxidation products 8-OH-dG and 5-OH-dC by 31.66%and 18.91%,respectively(P<0.01);increase the activity of antioxidant enzymes SOD and GSH-Px by 83.74%and 59.31%,respectively;and decrease NOX and PKC activity by 41.21%and 40.65%,respectively(P<0.01).Conclusion:Mulberry leaf alkaloids have a good effect on improving D-Gal-induced oxidative damage in the mouse kidney,and the underlying mechanism may be related to increasing the activity of SOD and GSH-Px and inhibiting the activity of NOX and PKC,thereby reducing the overproduction of reactive oxygen species(ROS)in the body.

关 键 词：桑叶生物碱 D-半乳糖 氧化应激 肾损伤 机制 

分 类 号：R285.5[医药卫生—中药学]