Preparation of pH-Responsive Doxorubicin Nanocapsules by Combining High-gravity Antisolvent Precipitation with In-situ Polymerization for Intracellular Anticancer Drug Delivery  

作　　者：LIU Jie CHEN Bo ZHANG Jianjun 

机构地区：[1]College of Chemical Engineering,Beijing University of Chemical Technology,Beijing 100029,P.R.China [2]State Key Laboratory of NBC Protection far Civilian,Beijing 102205,P.R.China

出　　处：《Chemical Research in Chinese Universities》2020年第5期927-933,共7页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(Nos.2147601&21622601);the National Key R&D Pro gram of China(No.2015CB932101).

摘　　要：Owing to the low pH value in tumor and cancer cells,drug delivery systems based on pH-responsive polymer nanocarriers have been extensively explored for anticancer chemotherapy.Herein,we developed a pH-responsive doxonibicin(EXDX)nanocapsule(named as DNanoCapsule)prepared by combining in-situ polymerization teclinique with high-gravity antisolvent precipitation technique throngh an amphiphilic polymerized surface ligand.DNanoCapsules show an obvious spherical core-shell structure with a single DOX nanoparticle encapsulated in the polymer layer.Dissolution rate studies prove that the DNanoCapsules have robust dnig-release profiles under acidic environments due to the division of the pH-sensitive cross-linker,which triggers thecollapse of the polymer layer.The in vitro investigations demonstrated that the DNanoCapsules exhibited higli cellular uptake efficiency and cytotoxicity for both HeLa and MCF-7 cancer cells.Tlierefore,this work may provide a promising strategy to design and develop various stimuli-responsive drug nanocapsules tor the treatment of cancer or other diseases.

关 键 词：PH-RESPONSIVE NANOCAPSULE In-situ polymerization High-gravity anti solvent precipitation Anticancer cheniotherapy 

分 类 号：TQ460.4[化学工程—制药化工]