基于ROS介导的自噬通路探讨补肾复方对AD秀丽隐杆线虫作用机制的研究进展  

Research progress of the effects of compound Chinese medicine for reinforcing kidney based on the ROS mediated autophagy pathway of AD C.elegans

作　　者：周玲 蔡铭 胡安娜 黄敏 彭圆 王平 ZHOU Ling;CAI Ming;HU An-na;HUANG Min;PENG Yuan;WANG Ping(Key Laboratory of Dementia on Xingnao Yizhi of National Administration of Traditional Chinese Medicine and Hubei University of Chinese Medicine,Institute of Gerontology,Hubei University of Chinese Medicine,Wuhan 430065,China)

机构地区：[1]湖北中医药大学国家中医药管理局醒脑益智重点实验室,湖北中医药大学老年医学研究所,武汉430065

出　　处：《中华中医药杂志》2020年第9期4558-4561,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81574037);湖北中医药大学青苗计划(No.2017ZZX007)。

摘　　要：阿尔茨海默病(AD)是一种难治性神经系统退行性疾病。将活性氧(ROS)介导的PI3K/AKT-mTOR自噬通路作为中药补肾复方治疗AD的重要靶点,选用秀丽隐杆线虫AD模型,利用高通量筛选确定药物抗氧化活性,结合ROS定量、RT-PCR和Western Blot等方法进行相关指标检测,深入探讨中医补肾复方抗AD的作用机制,为丰富和发展中医治则治法理论、寻找和开发临床新型防治AD药物提供科学依据。Alzheimer’s disease(AD)is one of the refractory neurodegenerative diseases.ROS mediated PI3 K/AKTmTOR autophagy pathways will be an important target of Chinese medicine reinforcing kidney in compound treatment of AD,applying the C.elegans AD model,high-throughput screening is used to determine the antioxidant activity,combined with ROS,quantitative RT-PCR and Western Blot test related indexes,discusses the mechanism of compound Chinese medicine for reinforcing kidney resistance to the AD,for the enrichment and development of theory of TCM,to find and provide a scientific basis for clinical prevention and treatment of AD new drug development.

关 键 词：老年性痴呆 秀丽隐杆线虫 ROS 自噬 

分 类 号：R285[医药卫生—中药学]