Proteome changes of porcine follicular fluid during follicle development  

作　　者：Victor M.Paes Shengfa F.Liao Jose R.Figueiredo Scott T.Willard Peter L.Ryan Jean M.Feugang 

机构地区：[1]Department of Animal and Dairy Sciences,Mississippi State University,4025 Wise Center,PO Box 9815,Starkville,Mississippi State MS 39762,USA [2]Laboratory of Manipulation of Oocyte and Preantral follicles,State University of Ceará,Fortaleza,CE,Brazil

出　　处：《Journal of Animal Science and Biotechnology》2020年第2期367-379,共13页畜牧与生物技术杂志（英文版）

基　　金：supported by the USDA-ARS Biophotonics(Grant#58–6402–3-018);the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior–Brasil(CAPES)–Finance Code 001.VM Paes is the recipient of a PhD scholarship from CAPES。

摘　　要：Background:Ovarian follicular fluid influences follicle and oocyte growth,but the fluctuation of its protein content during folliculogenesis has not been comprehensively analyzed.Here we used a shotgun approach and bioinformatics analyses to investigate and compare the proteomes of porcine follicular fluid(pFF)obtained from small(<4 mm),medium(4–6 mm)and large(>6–12 mm)follicles.Results:Follicular fluid samples containing highest estrogen levels were selected as non-atretic from small(SNA:26.1±15 ng/mL),medium(MNA:162±54 ng/mL),and large(LNA:290±37 ng/mL)follicles for proteomic analyses.We detected 1627,1699,and 1756 proteins in SNA,MNA,and LNA samples,respectively.Nearly 60–63%of total proteins were specific to each sample,11–13%were shared in pairwise comparisons,and 247 proteins were shared among all samples.Functional categorization indicated comparable gene ontology(GO)terms distribution per cellular component,molecular function,and biological process categories across samples;however,the ranking of highly significantly enriched GO terms per category revealed differences between samples.The patterns of proteinto-protein interactions varied throughout follicle development,and proteins such as serine protease inhibitor,clade E(SERPINE);plasminogen activator,urokinase(PLAU);and plasminogen activator,urokinase receptor(PLAUR)appeared stage-specific to SNA,MNA,and LNA,respectively.The“complement and coagulation cascades”was the common major pathway.Besides,properdin and fibulin-1 were abundant proteins that appeared absent in LNA samples.Conclusion:This study provides extensive and functional analyses of the pFF proteome changes during folliculogenesis and offers the potential for novel biomarker discovery in pFF for oocyte quality assessment.

关 键 词：Follicular fluid FOLLICULOGENESIS PIG Shotgun proteomic 

分 类 号：S828[农业科学—畜牧学]