Black soldier fly and gut health in broiler chickens:insights into the relationship between cecal microbiota and intestinal mucin composition  

作　　者：Ilaria Biasato Ilario Ferrocino Sihem Dabbou Rocchina Evangelista Francesco Gai Laura Gasco Luca Cocolin Maria Teresa Capucchio Achille Schiavone 

机构地区：[1]Department of Agricultural,Forest and Food Sciences,University of Turin,Largo Paolo Braccini 2,10095 Grugliasco,TO,Italy [2]Department of Veterinary Sciences,University of Turin,Largo Paolo Braccini 2,10095 Grugliasco,TO,Italy [3]Institute of Science of Food Production,National Research Council,Largo Paolo Braccini 2,10095 Grugliasco,TO,Italy

出　　处：《Journal of Animal Science and Biotechnology》2020年第3期841-852,共12页畜牧与生物技术杂志（英文版）

基　　金：Financial support for this work was provided by University of Turin(ex 60%)grant(Es.fin.2015-2016-2017).

摘　　要：Background:The relationship between diet and intestinal microbiota and mucin composition appears to be fundamental for poultry gut health.The effects of insect meal(whose role as alternative feed ingredient is now well recognized)on gut microbiota and mucin composition have recently been reported in Tenebrio molitor-fed free-range and broiler chickens,but no data are currently available for Hermetia illucens(HI)-fed broilers.The present study evaluated the effects of dietary HI meal inclusion on cecal microbiota and intestinal mucin composition of broiler chickens.Results:A total of 256 male broiler chickens were allotted to 4 dietary treatments(control diet[C]and 5%,10%and 15%HI meal inclusion,with 8 replicate pens/treatment and 8 birds/pen)and slaughtered at 35 d of age(2 animals/pen,16 birds/diet).The cecal microbiota assessment by 16S rRNA amplicon based sequencing showed lower alpha diversity in HI15 chickens(Shannon,P<0.05)and higher beta diversity(Adonis and ANOSIM,P<0.001)in birds fed HI diets than C.Furthermore,HI15 birds displayed significant increase of the relative abundance of Proteobacteria phylum(False Discovery Rate[FDR]<0.05)when compared to HI10.L-Ruminococcus(Ruminococcus from Lachnospiraceae family),Faecalibacterium,Blautia and Clostridium genera were found to be characteristic of HI5 cecal microbiota(FDR<0.05),while broiler chickens fed HI10 and HI15 diets were characterized(FDR<0.05)by Lactobacillus and Ruminococcus(HI10)and Bacteroides,Roseburia and Helicobacter genera(HI15).Periodic-acid Schiff,Alcian Blue pH 2.5 and high iron diamine staining on small and large intestine also demonstrated lower mucin staining intensity in the intestinal villi of HI10 and HI15 birds than C(P<0.05).Conclusions:Dietary HI meal utilization at low inclusion levels(i.e.,5%)positively influenced either the cecal microbiota or the gut mucin dynamics in terms of selection of potentially beneficial bacteria and increase in villi mucins.However,high inclusion levels(in particular the 15%)may have a negative influenc

关 键 词：16S rRNA Gut health Hermetia illucens L. HISTOCHEMISTRY Insect meal MICROBIOTA MUCIN POULTRY 

分 类 号：S831.5[农业科学—畜牧学]