食管鳞状细胞癌组织中miR-504和磷脂酰肌醇3-激酶/蛋白激酶B通路相关分子表达及其与患者临床病理学特征的关系  

Expression of miR-504 and phosphatidylinositol 3-kinase/protein kinase B pathway related molecules in esophageal squamous cell carcinoma and the relationship between them with the clinicopathological feature of patients

作　　者：任利兵[1] 贾如江[1] 苏春永[1] 杨晓光[1] 秦景云[1] 熊正明 REN Libing;JIA Rujiang;SU Chunyong;YANG Xiaoguang;QIN Jingyun;XIONG Zhengming(Department of Thoracic Surgery,Handan Central Hospital,Handan 056001,Hebei Province,China;Department of Oncology,Shijiazhuang Ping′an Hospital,Shijiazhuang 050000,Hebei Province,China)

机构地区：[1]邯郸市中心医院胸外科,河北邯郸056001 [2]石家庄平安医院肿瘤科,河北石家庄050000

出　　处：《新乡医学院学报》2020年第9期839-843,共5页Journal of Xinxiang Medical University

基　　金：河北省医学科学研究重点课题计划(编号:ZL20140229)。

摘　　要：目的探讨食管鳞状细胞癌组织中miR-504和磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)通路相关分子的表达及其与患者临床病理学特征的关系。方法选择2016年1月至2018年12月于邯郸市中心医院就诊并进行手术治疗的食管鳞状细胞癌患者67例为研究对象,取术中切除的新鲜食管鳞状细胞癌组织和距离癌组织5 cm以上正常食管鳞状上皮组织(癌旁组织),采用反转录-聚合酶链反应检测食管鳞状细胞癌组织和癌旁组织中miR-504表达,免疫组织化学法检测食管鳞状细胞癌组织及癌旁组织中PI3K、磷酸化Akt(p-Akt)表达;分析食管鳞状细胞癌组织中miR-504、PI3K、p-Akt表达与患者临床病理学特征的关系及miR-504与PI3K、p-Akt表达的相关性。结果miR-504在癌组织中的表达量显著低于癌旁组织(P<0.05),miR-504在不同分化程度癌组织中的表达量两两比较差异均有统计学意义(P<0.05),miR-504在Ⅰ期患者癌组织中表达量显著高于Ⅱ、Ⅲ期患者(P<0.05)。PI3K、p-Akt在癌组织中表达评分显著高于癌旁组织(P<0.05)。PI3K、p-Akt在不同分化程度癌组织中的表达量两两比较差异均有统计学意义(P<0.05)。PI3K、p-Akt在Ⅱ、Ⅲ期患者癌组织中表达量显著高于Ⅰ期患者(P<0.05)。食管鳞状细胞癌组织中miR-504、PI3K、p-Akt的表达与患者年龄、性别无关(P>0.05),与癌组织的分化程度、TNM分期和淋巴结转移相关(P<0.05)。食管鳞状细胞癌组织中miR-504表达与PI3K、p-Akt表达呈明显负相关(r=-0.354、-0.519,P<0.05)。结论miR-504在食管鳞状细胞癌组织中的表达与肿瘤的分化程度、TNM分期和淋巴结转移有关,其在食管鳞状细胞癌中可能作为抑癌基因发挥作用,且这种抑制作用可能与抑制肿瘤细胞内PI3K/Akt信号通路的活性有关。Objective To investigate the expression of miR-504,phosphatidylinositol 3-kinase/protein kinase B(PI3K/Akt)pathway related molecules in esophageal squamous cell carcinoma tissue and the relationship between them with the clinicopathological feature of patients.Methods A total of 67 patients with esophageal squamous cell carcinoma who were treated in Handan Central Hospital from January 2016 to December 2018 were enrolled in this study.The fresh esophageal squamous cell carcinoma tissue and normal esophageal squamous epithelial tissues which were away from cancer tissue more than 5 cm(paracancerous tissues)were taken during the operation.The expression of miR-504 in esophageal squamous cell carcinoma and paracancerous tissues was detected by reverse transcription polymerase chain reaction.The expression of PI3K and phosphorylated Akt(p-Akt)in esophageal squamous cell carcinoma and paracacerous tissues was detected by immunohistochemistry.The relationship between the expression of miR-504,PI3K,p-Akt and the clinicopathological characteristics of patients and the correlation between the expression of miR-504 and PI3K,p-Akt were analyzed.Results The expression of miR-504 in cancer tissues was significantly lower than that in the paracacerous tissues(P<0.05);there was statistic difference in the expression of miR-504 in cancer tissues with different degrees of differentiation(P<0.05);the expression of miR-504 in cancer tissues of stageⅠpatients was significantly higher than that of stageⅡ,Ⅲpatients(P<0.05).The scores of PI3K,p-Akt expression in cancer tissues were significantly higher than those in the paracancerous tissues(P<0.05).There was statistic difference in the expressions of PI3K,p-Akt in cancer tissues with different degrees of differentiation(P<0.05);the expressions of PI3K,p-Akt in cancer tissues of stageⅡ,Ⅲpatients were significantly higher than those of stageⅠpatients(P<0.05).The expressions of miR-504,PI3K,p-Akt in esophageal squamous cell carcinoma were not correlated with age and gender of

关 键 词：食管鳞状细胞癌 miR-504 磷脂酰肌醇3-激酶/蛋白激酶B信号通路 

分 类 号：R735.1[医药卫生—肿瘤]