重离子辐照对盐生杜氏藻的诱变效应及光合响应机理  

Photosynthetic profiling and response to heavy-ion irradiation in Dunaliella salina

作　　者：席一梅 殷亮 迟占有[2] 李欣[3] 罗光宏[1] XI Yimei;YIN Liang;CHI Zhanyou;LI Xin;LUO Guanghong(Hexi University,Gansu Microalgae Technology Innovation Center,Zhangye 734000,China;School of Bioengineering,Dalian University of Technology,Dalian 116024,China;Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,China)

机构地区：[1]河西学院甘肃省微藻技术创新中心,张掖734000 [2]大连理工大学生物工程学院,大连116024 [3]中国科学院近代物理研究所,兰州730000

出　　处：《辐射研究与辐射工艺学报》2020年第5期34-41,共8页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(11665011);甘肃省高校协同创新科技团队支持计划(2017C-17);甘肃省科技计划项目(18JR2JG001)资助。

摘　　要：为探讨重离子束对盐生杜氏藻(Dunaliella salina)的生物效应及光合响应机制,本研究利用0~320 Gy剂量范围内的重离子对盐生杜氏藻进行辐照处理,并对重离子辐照后10 d内的藻细胞生物量、叶绿素荧光参数及β-胡萝卜素的积累量等进行了系统的测定和分析。对细胞干重的测定结果表明:与未辐照盐生杜氏藻相比,90 Gy、240 Gy和320 Gy的重离子辐照均显著提升了藻细胞10 d内的生物量,240 Gy辐照后的藻细胞生物量最大提升了1.78倍;进一步应用叶绿素荧光成像系统Water-PAM对辐照后藻细胞的叶绿素荧光参数,包括光系统Ⅱ(PSⅡ)的光化学效率(Fv/Fm)、实际光合效率(ΦPSII)及热耗散能力(NPQ)等,指标的变化趋势进行分析。表明上述3个剂量的辐照有效提升了盐生杜氏藻的光合作用效率,其中240 Gy辐照可以使藻细胞更快地启动光保护机制。通过紫外分光光度法对辐照后β-胡萝卜素的积累进行了检测分析,结果表明,利用240 Gy和320 Gy的重离子辐照10 d后,β-胡萝卜素产量分别提高了1.3倍和1.1倍。本研究首次探讨了重离子辐照的剂量和时间对盐生杜氏藻的生物效应,同时也为绿藻光合系统对辐照损伤的响应机制提供了初步的理论和实验依据。In this study,we aimed to explore the biological effect and photosynthetic response mechanism of heavy-ion irradiation on Dunaliella salina.Dunaliella salina was treated with different doses(0~320 Gy)of heavyion irradiation.The cell biomass,chlorophyll fluorescence parameters,and main photosynthetic pigments of progenies from irradiated Dunaliella salina cells were systematically measured and analyzed within 10 days of treatment.The results showed a significant increase in the biomass of cells that underwent heavy-ion irradiation at 90 Gy,240 Gy,and 320 Gy doses compared with non-irradiated Dunaliella salina cells.In particular,the maximum biomass of irradiated cells was 1.78-fold greater than of non-irradiated cells at 240 Gy.The chlorophyll fluorescence imaging system,Water-PAM,was used to analyze the chlorophyll fluorescence parameters,including photochemical efficiency of photosystem II(Fv/Fm),actual photosynthetic activity(ΦPSII),and non-photochemical quenching capacity(NPQ)of the cells after irradiation.The results indicated that 90 Gy,240 Gy,and 320 Gy doses notably increased the photosynthetic efficiency of Dunaliella salina.The 240 Gy dose-irradiated algal cells exhibited activated photoprotection systems more rapidly as compared to cells irradiated at other doses.Additionally,theβ-carotene content of the algal cells after irradiation was measured using UV spectrophotometry.The results revealed thatβ-carotene production increased compared with non-irradiated Dunaliella salina cells,by 1.3 and 1.1 times with 240 Gy and 320 Gy,respectively.To our knowledge,this study investigated the dose and time response to heavy-ion irradiation on Dunaliella salina mutagenesis for the first time,and provided a preliminary theoretical and experimental basis for the response mechanism of the photosynthetic system to heavy-ion irradiation.

关 键 词：重离子辐照 盐生杜氏藻 光合效率 Β-胡萝卜素 

分 类 号：Q691[生物学—生物物理学]