超高效液相色谱-串联质谱法测定畜禽副产品中氯霉素和氟甲砜霉素残留量  

Simultaneous determination of chloramphenicol and florfenicol residues in animal by-products with ultra-performance liquid chromatography-tandem mass spectrometry

作　　者：袁潇 王淼 叶一 王丽敏 赖晨焕 陈超薇 YUAN Xiao;WANG Miao;YE Yi;WANG Limin;LAI Chenhuan;CHEN Chaowei(Chengdu Institutes for Food and Drug Control,Chengdu 610044;Chengdu Testing Center of Agriculture Quality Supervision,Chengdu610041)

机构地区：[1]成都市食品药品检验研究院,成都610044 [2]成都市农业质量监测中心,成都610041

出　　处：《分析试验室》2020年第9期1094-1098,共5页Chinese Journal of Analysis Laboratory

摘　　要：建立了超高效液相色谱-串联质谱测定畜禽副产品中氯霉素和氟甲砜霉素残留量的方法。样品经碱性乙酸乙酯提取,正己烷脱脂后,直接采用电喷雾电离(ESI)多反应监测(MRM)负离子模式检测,氯霉素用同位素内标定量,氟甲砜霉素用外标法定量。氯霉素和氟甲砜霉素在0.2~50μg/L范围内线性良好,相关系数达到0.9999;检出限均为0.04μg/kg;氯霉素回收率为89.6%~112.0%,RSD为5.4%~8.1%;氟甲砜霉素回收率为90.3%~110.0%,RSD为5.1%~8.0%。方法可用于畜禽副产品中氯霉素和氟甲砜霉素残留量测定。A method for the determination of chloramphenicol( CPA) and florfenicol( FF) in animal byproducts by ultra-performance liquid chromatography-tandem mass spectrometry( UPLC-MS/MS) was established. Samples were extracted with alkaline ethyl acetate and degreased with n-hexane. The targets were directly detected by MS/MS in negative electrospray ionization( ESI) and multiple reaction monitoring( MRM)mode. Chloramphenicol was quantified with isotopic internal standard and florfenicol was quantified by external standard. The calibration curve showed a good linearity in the range of 0. 2-50. 0 μg/L with the correlation coefficient r > 0. 9999. The detection limits for chloramphenicol and florfenicol were both 0. 04 μg/kg. The average recoveries of chloramphenicol were 89. 6%-112. 0% with RSD of 5. 4%-8. 1% and the average recoveries of florfenicol were 90. 3%-110. 0% with RSD of 5. 1%-8. 0%. This method is suitable for simultaneous determination of chloramphenicol and florfenicol residues in animal by-products.

关 键 词：UPLC-MS/MS 畜禽副产品 氯霉素 氟甲砜霉素 

分 类 号：TS207.3[轻工技术与工程—食品科学]