TDP-43、LC3在多发性肌炎及散发性包涵体肌炎中的表达及其作用  

Expression and role of TDP-43 and LC3 in polymyositis and sporadic inclusion body myositis

作　　者：曾国勇[1] 曾祥俊[1] 叶瑾[2] ZENG Guo-yong;ZENG Xiang-jun;YE Jin(Department of Neurology,Ganzhou People′s Hospital,Jiangxi Province,Ganzhou341000,China;Department of Nephrology,Ganzhou People′s Hospital,Jiangxi Province,Ganzhou341000,China)

机构地区：[1]江西省赣州市人民医院神经内科,江西赣州341000 [2]江西省赣州市人民医院肾内科,江西赣州341000

出　　处：《中国当代医药》2020年第28期62-64,F0004,共4页China Modern Medicine

基　　金：江西省卫生计生委科技计划项目(20164060)。

摘　　要：目的探究TDP-43、LC3在多发性肌炎(PM)及散发性包涵体肌炎(IBM)中的表达及其作用。方法选择2018年3月~2019年9月我院收治的PM(26例)、IBM(26例)患者的中等受累肌肉进行活检,并避开肌电图穿刺点。将PM患者取出肌肉组织作为PM组,将IBM患者取出肌肉组织作为IBM组。通过免疫组化法、Western blot法等方法分析PM组和IBM组TDP-43、LC3阳性表达率及其蛋白含量的差异性,进而探究TDP-43、LC3在PM及IBM中的表达及其作用。结果免疫组化法结果显示,IBM组肌肉组织中TDP-43、LC3阳性表达高于PM组;Western blot法结果显示,IBM组肌肉组织中TDP-43、LC3蛋白含量高于PM组,差异有统计学意义(P<0.05)。结论TDP-43、LC3更易于在IBM骨骼肌标本中表达,可作为与PM鉴别诊断的指标之一。Objective To explore the expression and role of TDP-43 and LC3 in polymyositis(PM)and sporadic inclusion body myositis(IBM).Methods From March 2018 to September 2019,the moderately affected muscles of PM(26 cases)and IBM(26 cases)who were admitted to our hospital were selected for biopsy,and the electromyographic puncture points were avoided.The muscle tissue of the PM patients was taken as the PM group,and the muscle tissue of the IBM patients was taken as the IBM group.The differences of TDP-43,LC3 positive expression rate and protein content between PM group and IBM group by immunohistochemistry,Western blot and other methods were analyzed,and then the expression and role of TDP-43,LC3 in PM and IBM was explored.Results The results of immunohistochemistry showed that the positive expression of TDP-43 and LC3 in the muscle tissue of the IBM group was higher than that of the PM group;the results of Western blot showed that the protein contents of TDP-43 and LC3 in the muscle tissue of the IBM group were higher than those of the PM group,the differences were statistically significant(P<0.05).Conclusion TDP-43 and LC3 are more easily expressed in IBM skeletal muscle specimens,and can be used as one of the indicators for differential diagnosis from PM.

关 键 词：多发性肌炎 TDP-43 包涵体肌炎 LC3 

分 类 号：R746[医药卫生—神经病学与精神病学]