多通道电化学基因传感阵列快速联检AML相关耐药基因的新方法  

A novel method about multichannel electrochemical gene sensor array for rapid simultaneous detection of AML drug-resistant genes

作　　者：王昆[1] 张书仪 颜志文[1] 魏吟秋[1] 李碧艳[1] 陈方 陈庆[2] WANG Kun;ZHANG Shuyi;YAN Zhiwen;WEI Yinqiu;LI Biyan;CHEN Fang;CHEN Qing(Department of Pharmacy,the First Affiliated Hospital of Xiamen University,Xiamen,Fujian 361003,China;Faculty of Pharmacy,Xiamen University,Xiamen,Fujian 361102,China)

机构地区：[1]厦门大学附属第一医院药剂科,福建厦门361003 [2]厦门大学药学院,福建厦门361102

出　　处：《福州大学学报（自然科学版）》2020年第5期673-678,共6页Journal of Fuzhou University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(81903585);福建省卫生计生中青年骨干人才培养项目(2017-ZQN-85);厦门市科技计划(科技惠民)项目(3502Z20164001)。

摘　　要：筛选并制备高特异性DNA探针,与纳米粒子标记技术相结合,设计多通道电化学基因传感阵列检测模式,建立灵敏、快速、简便、经济的急性髓系白血病(AML)相关多药耐药基因(MDR1和MRP)检测新方法.该方法具有较好的特异性、灵敏度和重现性,能够在1.0×10^-14~1.0×10^-12 mol·L^-1和5.0×10^-14~5.0×10^-12 mol·L^-1范围内,分别实现对AML相关多药耐药基因序列MDR1和MRP的定量检测.该方法有望为预测肿瘤治疗效果和临床制定治疗方案提供参考依据.A sensitive,fast,simple and economic new method was established for simultaneous detection of acute myeloid leukemia(AML)related multidrug resistance genes(MDR1 and MRP)by screening of preparation about the high specific DNA probes and designing a multichannel electrochemical gene sensor array mode with nanoparticle labeling technology.The method can realize quantitative detection for MDR1 from 1.0×10^-14 to 1.0×10^-12 mol·L^-1 and MRP from 5.0×10^-14 to 5.0×10^-12 mol·L^-1,respectively.The method is expected to provide a reference for predicting the treatments of cancer and making clinical treatment strategies.

关 键 词：多通道电化学传感阵列 急性髓系白血病 多药耐药基因 

分 类 号：R-331[医药卫生]