miR-664b-5p对食管癌细胞EC9706和TE-1生物学行为的影响  

Effect of miR-664b-5p on the biological behavior of esophageal cancer cell lines EC9706 and TE-1

作　　者：向辉 唐明 李阳 林怡秀 郑勇[1] 陈卫刚[1] Xiang Hui;Tang Ming;Li Yang(Dept of Gastroenterology,The First Affiliated Hospital,School of Medicine,Shihezi University,Shihezi 832008)

机构地区：[1]石河子大学医学院第一附属医院消化内科,石河子832008

出　　处：《安徽医科大学学报》2020年第11期1746-1753,共8页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81260362);石河子大学成果转化与技术推广计划(编号:CGZH201704)。

摘　　要：目的探讨miR-664b-5p对食管癌细胞EC9706和TE-1生物学行为的影响。方法在人食管癌细胞株EC9706和TE-1转染miR-664b-5p mimic或inhibitor后,采用实时荧光定量聚合酶链式反应(qRT-PCR)检测细胞中miR-664b-5p的表达情况;通过CCK-8和平板克隆形成实验检测细胞增殖能力;通过Transwell实验检测细胞的迁移和侵袭能力;通过Western Blot检测细胞凋亡相关蛋白Bax和Bcl-2的表达。结果转染miR-664b-5p mimic后,食管癌细胞EC9706和TE-1中miR-664b-5p的表达增加(P<0.05),转染miR-664b-5p inhibitor后,miR-664b-5p的表达降低(P<0.05);增殖实验结果表明,与mimic NC组比较,miR-664b-5p mimic组食管癌细胞增殖能力下降,克隆形成数目减少(P<0.05),miR-664b-5p inhibitor组较inhibitor NC组食管癌细胞增殖能力增强,克隆形成数目增多(P<0.05);Transwell实验结果表明,miR-664b-5p mimic组食管癌细胞迁移和侵袭数目少于mimic NC组(P<0.05);miR-664b-5p inhibitor组食管癌细胞迁移和侵袭数目多于inhibitor NC组(P<0.05);Western blot实验结果表明,miR-664b-5p mimic组食管癌细胞Bax表达增加,Bcl-2表达下降(P<0.05),而miR-664b-5p inhibitor组Bax表达下降,Bcl-2表达增加(P<0.05)。结论 miR-664b-5p可以抑制食管癌细胞EC9706和TE-1的增殖、迁移和侵袭,促进细胞凋亡,为进一步研究miR-664b-5p在哈萨克族食管鳞癌中的分子机制提供了基础。Objective To investigate the effect of miR-664 b-5 p on the biological behavior of esophageal cancer cells EC9706 and TE-1.Methods Quantitative real-time PCR(qRT-PCR)was used to determine the expression of miR-664 b-5 p after transfected with miR-664 b-5 p mimic or inhibitor in esophageal cancer cells EC9706 and TE-1;Cell proliferation was assessed by CCK-8 and colony formation;Migration and invasion of esophageal cancer cells were observed by the transwell chamber assay;The expression of apoptosis-related proteins Bax and Bcl-2 were detected by Western blot.Results After the transfection of miR-664 b-5 p mimic,the expression level of miR-664 b-5 p increased in esophageal cancer cells EC9706 and TE-1(P<0.05),while the transfection of miR-664 b-5 p inhibitor was the opposite(P<0.05);The results of cell proliferation assay showed that the ability of the cell proliferation and the number of colony formation decreased in the miR-664 b-5 p mimic group compared with the mimic NC group(P<0.05),and the ability of the cell proliferation and the number of colony formation in the miR-664 b-5 p inhibitor group was higher than the inhibitor NC group(P<0.05);The results of transwell assay showed that the number of migration cells and invasive cells of esophageal cancer in the miR-664 b-5 p mimic group was lower than that of the mimic NC group(P<0.05),while the number of migration cells and invasive cells in the miR-664 b-5 p inhibitor group was higher than that of the inhibitor NC group(P<0.05);The results of Western blot showed that the expression of Bax increased and Bcl-2 reduced in the miR-664 b-5 p mimic group(P<0.05),but the expression of Bax reduced and Bcl-2 increased in the miR-664 b-5 p inhibitor group(P<0.05).Conclusion miR-664 b-5 p can inhibit cell proliferation,migration and invasion,and promote the apoptosis of esophageal cancer cells EC9706 and TE-1,which provides the foundation to further study the molecular mechanism of miR-664 b-5 p in Kazakh patients with esophageal squamous cell carcinoma.

关 键 词：miR-664b-5p 食管鳞癌 增殖 迁移 侵袭 凋亡 

分 类 号：R735.1[医药卫生—肿瘤]