三叉神经痛模型大鼠的三叉神经节中JAK/STAT3通路的活化  

Activation of the JAK/STAT3 pathway in the trigeminal ganglion of CCI-ION model rats

作　　者：张瑶 刘亚军 李芳 朱大卫 王元银[1] 侯爱兵[1] Zhang Yao;Liu Yajun;Li Fang(Stomatological College of Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学口腔医学院,合肥230032

出　　处：《安徽医科大学学报》2020年第10期1487-1490,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81271162);安徽省科技攻关计划项目(编号:1401045013);安徽省重点研究和开发计划项目(编号:201904a07020062);安徽省重点研究与开发计划项目(编号:1704f0804025)。

摘　　要：目的检测用眶下神经缩窄术建立的大鼠三叉神经痛(TN)模型中三叉神经节(TG)内pSTAT3蛋白表达的变化及其定位的细胞类型。方法随机将成年雄性SD大鼠分成两组:眶下神经缩窄术组(CCI-ION组)和假手术组(Sham组),用Von Frey毛刷检测两组大鼠的机械敏感阈值。通过Western blot检测术后TG内pSTAT3蛋白表达量变化,并用免疫荧光实验观察pSTAT3的荧光染色情况及其定位细胞类型。结果CCI-ION组大鼠在术后第1~14天出现机械敏感阈值的显著降低(P<0.05)。CCI-ION组内大鼠TG的pSTAT3蛋白表达量相较于Sham组大鼠明显升高(P<0.05)。CCI-ION组大鼠pSTAT3的荧光染色亦明显强于Sham组,行免疫荧光双染实验发现pSTAT3定位于大鼠TG的小胶质细胞内而非卫星胶质细胞或神经元细胞内。结论在TN模型大鼠的TG小胶质细胞内有JAK/STAT3通路的活化。Objective To detect the expression changes of pSTAT3 protein in trigeminal ganglions(TGs) of trigeminal neuralgia(TN) model rats and its located cell type. Methods The rat model of TN was established by chronic constriction of infraorbital nerve. Von Frey hair was used to determine the mechanical sensitivity threshold of the two groups, and Western blot was used to detect the changes of pSTAT3 protein expression in TG. Immunofluorescence assay was performed to observe the fluorescence staining of pSTAT3 and its located cell type. Results The CCI-ION group showed a significant reduction of the mechanical pain threshold(P<0.05) from the first day to the fourteenth day after surgery. The expression level of pSTAT3 protein in TG of CCI-ION group was significantly higher than that of Sham group(P<0.05). The immunofluorescence experiment showed that the staining of pSTAT3 in the CCI-ION group was stronger than that in the Sham group, and pSTAT3 was located in microglia cells rather than satellite glia cells or neurons. Conclusion The activation of JAK/STAT3 pathway was found in the TG microglias of TN model rats.

关 键 词：三叉神经痛 眶下神经缩窄术 JAK/STAT3 pSTAT3 三叉神经节 

分 类 号：R745.11[医药卫生—神经病学与精神病学]