子宫颈鳞状细胞癌患者SFRP基因甲基化与临床病理特征及HPV感染的关系  

Relationship between Methylation of SFRP Gene and Clinicopathological Features and HPV Infection in Patients with Cervical Squamous Cell Carcinoma

作　　者：徐志红[1] 邓莹[2] XU Zhihong;DENG Ying(Department of Reproductive Genetics,People's Hospital of Deyang City,Deyang 618000,Sichuan,China;National Center for Birth Defect Monitoring,West China Second University Hospital,Sichuan University,Chengdu 610041,Sichuan,China)

机构地区：[1]德阳市人民医院生殖遗传科,四川德阳618000 [2]四川大学华西第二医院中国出生缺陷监测中心,四川成都610041

出　　处：《贵州医科大学学报》2020年第10期1214-1218,共5页Journal of Guizhou Medical University

基　　金：四川省卫生厅科研课题(130439)。

摘　　要：目的:探讨子宫颈鳞状细胞癌患者分泌型卷曲相关蛋白(SFRP)基因甲基化与临床病理特征及人乳头瘤病毒(HPV)感染的关系。方法:选取87例子宫颈鳞状细胞癌患者术中切除的癌组织及配对癌旁正常组织,采用甲基化特异性聚合酶链式反应(MSP)法检测组织中SFRP基因甲基化率,采用实时荧光定量聚合酶链反应(q-PCR)检测子宫颈鳞状细胞癌患者HPV感染情况,采用S pearman相关分析子宫颈鳞状细胞癌患者SFRP基因甲基化水平与其临床病理特征及HPV感染的相关性。结果:子宫颈鳞状细胞癌组织中SFRP基因甲基化率高于癌旁正常组织,差异有统计学意义(P<0.05);不同年龄、肿瘤直径、淋巴结转移、浸润深度的子宫颈鳞状细胞癌患者癌组织SFRP基因甲基化率比较,差异无统计学意义(P>0.05);不同WHO病理分级、FIGO分期的子宫颈鳞状细胞癌患者癌组织SFRP基因甲基化率比较,差异具有统计学意义(P<0.05);HPV感染阳性子宫颈鳞状细胞癌患者癌组织SFRP基因甲基化率显著高于HPV感染阴性子宫颈鳞状细胞癌患者,差异有统计学意义(P<0.05);SFRP基因甲基化率与子宫颈鳞状细胞癌患者年龄、肿瘤直径、淋巴结转移、浸润深度无相关性(P>0.05),与子宫颈鳞状细胞癌患者的WHO病理分级、FIGO分期、HPV感染呈正相关(r=0.445、0.305、0.260,P<0.05)。结论:SFRP基因甲基化率与子宫颈鳞状细胞癌患者的WHO病理分级、FIGO分期、HPV感染呈正相关,有望成为子宫颈鳞状细胞癌诊断的生物标志物。Objective:To investigate the relationship between methylation of secreted frizzled-related protein(SFRP)gene and clinicopathological features and human papillomavirus(HPV)infection in patients with cervical squamous cell carcinoma.Methods:87 patients with cervical squamous cell carcinoma were enrolled;cancer tissues and adjacent normal tissues were collected.The methylation rate of SFRP gene was detected by methylation specific polymerase chain reaction(MSP);Real time fluorescence quantitative polymerase chain reaction(q-PCR)was used to detect HPV infection in patients with cervical squamous cell carcinoma;Spearman correlation was used to analyze the correlation between the methylation rate of SFRP gene and the clinicopathological characteristics and HPV infection.Results:The methylation rate of SFRP gene in cancer tissues was significantly higher than that in adjacent normal tissues(P<0.05).The methylation rate of SFRP gene had no significant difference among patients with different age,tumor diameter,lymph node metastasis and invasion depth(P>0.05),while had significant difference among patients with different WHO grades and FIGO stages(P<0.05).The methylation rate of SFRP gene in HPV positive patients was higher than that of HPV negative patients(P<0.05).The methylation rate of SFRP gene was not correlated with age,tumor diameter,lymph node metastasis and invasion depth(P>0.05),while was positively correlated with WHO grades,FIGO stages and HPV infection(r=0.445,0.305,0.260,P<0.05).Conclusion:The methylation rate of SFRP gene has positive correlation with the WHO pathological grade,FIGO stage and HPV infection in patients with cervical squamous cell carcinoma,which is expected to be a biomarker for clinical diagnosis.

关 键 词：癌 鳞状细胞 子宫颈鳞状细胞癌 分泌型卷曲相关蛋白 基因甲基化 临床病理特征 人乳头瘤病毒 

分 类 号：R737.33[医药卫生—肿瘤]