诱导型一氧化氮合成酶在胃腺癌表达特点及与淋巴管形成的关系  

Expression of iNOS and D2-40 protein in gastric adenocarcinoma and its significance in lymphangiogenesis

作　　者：王嘉鑫 闫志军 石安华 刘宥苡 吕煜 陈芸[4] 代佑果[1] WANG Jia-xin;YAN Zhi-jun;SHI An-hua;LIU You-yi;LU Yu;CHEN Yun;DAI You-guo(Adominal Surgery,the third Affilliated Hospital of Kunming Medical University,Kunming 650118,Yunnan,China;Department of Surgery,Shenzhen people's Hospital,Shenzhen 518020,Guangdong,China;Department of Pathology,Yunnan University of Traditional Chinese Medicine,Kunming 650500,Yunnan,China;Department of Pathology,the third Affiliated Hospital of Kunming Medical University,Kunming 650118,Yunnan,China)

机构地区：[1]昆明医科大学第三附属医院/云南省肿瘤医院胃与小肠外科,昆明650118 [2]深圳市人民医院外科,深圳518020 [3]云南中医药大学病理教研室,昆明650500 [4]昆明医科大学第三附属医院/云南省肿瘤医院病理科,昆明650118

出　　处：《医学研究生学报》2020年第9期937-941,共5页Journal of Medical Postgraduates

基　　金：云南省科技厅.昆明医科大学联合专项基金(2018FE001-064);昆明医科大学第三附属医院博士科研基金(BSKY201707)。

摘　　要：目的诱导型一氧化氮合成酶(iNOS)表达与胃癌淋巴管形成及淋巴结转移的关系研究较少。文中旨在探讨胃腺癌组织中iNOS的表达,及与淋巴管形成的相关性。方法收集2017年7月至2018年12月昆明医科大学第三附属医院腹部外科78例胃腺癌并行胃腺癌根治术患者的癌组织及癌旁组织标本。采用免疫组化法检测iNOS和D2-40蛋白的表达,分析iNOS与D2-40蛋白表达的相关性,分析iNOS表达与胃腺癌临床病理特征的关系。结果胃腺癌组织中iNOS阳性表达率为64.10%(50/78),D2-40阳性表达率为73.08%(57/78)。胃腺癌组织的iNOS、D2-40阳性表达率明显高于癌旁组织(P<0.05)。胃腺癌组织中iNOS和D2-40的表达呈显著正相关(rs=0.532,P=0.001)。胃腺癌组织中iNOS表达与肿瘤分化程度有关,低分化腺癌阳性表达率较高中分化明显升高(72%vs 28%,P=0.041),iNOS阳性表达率随着临床T分期和N分期越晚越高(P<0.05)。结论 iNOS表达在胃癌的淋巴管的形成中起重要作用,iNOS蛋白具有潜在靶向治疗的价值。Objective To investigate the expression of iNOS in gastric adenocarcinoma tissues and its correlation withlymphangiogenesis. Methods The cancer tissue samples and adjacent tissue samples of78 clinically diagnosed gastric adenocarcinoma patients undergoing radical gastrectomy were collected. The expression of iNOS and D2-40 was detected by immunohistochemistryinAbdominal Surgery, the Third Affiliated Hospital of Kunming Medical University from July, 2017 to December, 2018. The correlation between iNOS and D2-40 protein expression was analyzed, and the relationship between the expression of iNOS and the clinicopathological characteristics of gastric adenocarcinoma was analyzed. Results iNOS expression was detected in 50 patients with gastric adenocarcinoma. The positive expression rate was 64.10%(50/78), of which 11 were low, 18 were middle and 21 were high. The positive expression rate of iNOS in gastric adenocarcinoma tissues was higher than that in paracancerous tissues, and the difference was statistically significant(P= 0.014). D2-40 expression was detected in 57 patients with gastric adenocarcinoma with a positive expression rate of 73.08%(57/78), of which 13 had low expression, 20 in medium and 24 in high expression. The positive expression rate of D2-40 in gastric adenocarcinoma tissues was higher than that in adjacent tissues, and the difference was statistically significant(P=0.003). The expression of iNOS and D2-40 was significantly positively correlated in gastric adenocarcinoma tissues(P=0.001, r=0.532). The expression of iNOS in gastric adenocarcinoma tissues was related to the degree of tumor differentiation, and the low-differentiated adenocarcinoma showed a higher positive expression rate than high-grade differentiated adenocarcinoma(P=0.041). In addition, the positive expression rate of iNOS increased with clinical T staging(P=0.043) and N staging(P=0.005) later. Conclusion iNOS was highly expressed in gastric adenocarcinoma tissues, and had a significant correlation with tissue differentiation, T

关 键 词：胃腺癌 诱导型一氧化氮合酶 D2-40 淋巴管形成 

分 类 号：R735.2[医药卫生—肿瘤]