L1细胞黏附分子在结肠癌中表达的临床意义和生物学作用  

Clinical significance and biological role of L1 cell adhesion molecule in colon cancer

作　　者：陈立智 胡军 傅厚丰 符敏 CHEN Lizhi;HU Jun;FU Houfeng;FU Min(Department of General Surgery,Qionghai People′s Hospital Hospital,Qionghai 571400,China)

机构地区：[1]琼海市人民医院普通外科,海南琼海571400

出　　处：《实用医学杂志》2020年第19期2690-2695,共6页The Journal of Practical Medicine

基　　金：琼海市科技研发项目。

摘　　要：目的探讨L1细胞黏附分子(L1 cell adhesion molecules,L1CAM)在结肠癌(colon cancer,CC)中表达的临床意义及生物学作用。方法采用免疫组化法检测166例CC癌和癌旁组织中L1CAM蛋白表达情况,分析L1CAM表达与CC患者临床病理特征的关系和独立预后风险因素,绘制L1CAM高低表达组患者的总体生存曲线;选取CC细胞系HCT116和LS174T,以及正常结肠上皮细胞系FHC和NCM460。利用小分子RNA沉默L1CAM表达,探讨L1CAM在CC细胞中的生物学作用。结果CC组织中LICAM阳性率为75.30%,显著高于癌旁组织的阳性率24.70%(P<0.05);L1CAM表达与CC患者肿瘤直径、分化程度、淋巴转移和临床分期情况显著相关(P<0.05);其中,相比于淋巴结未转移的患者,淋巴结转移组的患者的组织中L1CAM蛋白的阳性率更高(P<0.05)。在CC细胞系HCT116和LS174T中L1CAM高表达(P<0.05);在敲除HCT116和LS174T细胞L1CAM蛋白后,细胞的迁移能力显著降低(P<0.05),凋亡率显著升高(P<0.05),细胞增殖率显著下降(P<0.05)。结论L1CAM蛋白在人CC组织中高表达,提示患者预后不良,L1CAM蛋白可作为临床CC诊断和预后的一个分子指标。Objective This research tried to investigate the clinical significance and biological role of L1 cell adhesion molecule(L1 CAM)in colon cancer(CC).Methods Immunohistochemical method was applied to detect the expression of L1 CAM protein in 166 cases of CC cancer tissues and their adjacent tissues.The relationship between LICAM expression and clinicopathological characteristics of CC patients were explored.Independent prognostic risk factors were then analyzed.The overall survival rates of patients with high or low expression L1 CAM were analysed.In addition,the si-RNA of L1 CAM were transfected into CC cell lines(HCT116,LS174 T)and normal colonic epithelial cell lines(FHC,NCM460)to silence L1 CAM expression.The biological roles of L1 CAM in CC cells were explored.Results The positive rate of LICAM in CC tissue was 75.30%,which was significantly higher than the positive rate of 24.70%in adjacent tissues(P<0.05).L1 CAM expression was significantly correlated with tumor diameter,degree of differentiation,lymphatic metastasis and clinical stage of patients with CC(P<0.05).Moreover,the positive rate of L1 CAM protein in tissues of patients with lymph node metastasis was higher when comparing with patients without lymph node metastasis(P<0.05).It was also found that L1 CAM was highly expressed in CC cell lines HCT116 and LS174 T(P<0.05).After knocking out L1 CAM expression in HCT116 and LS174 T cells,the cell migration ability was obviously reduced(P<0.05)and the apoptosis rate was significantly increased(P<0.05).The cell proliferation rate decreased significantly(P<0.05).Conclusion LICAM protein was highly expressed in human CC tissues and it might be a poor prognosis indicator.L1 CAM protein could be used as a potential molecular indicator for clinical CC diagnosis and prognosis.

关 键 词：L1细胞黏附分子 结肠癌 预后 免疫组织化学 凋亡 

分 类 号：R735.35[医药卫生—肿瘤]